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. 1987 Nov;84(22):7896-900.
doi: 10.1073/pnas.84.22.7896.

Selective elimination of mRNAs in vivo: complementary oligodeoxynucleotides promote RNA degradation by an RNase H-like activity

P Dash  1 , I Lotan, M Knapp, E R Kandel, P Goelet
Affiliations

Selective elimination of mRNAs in vivo: complementary oligodeoxynucleotides promote RNA degradation by an RNase H-like activity

P Dash et al. Proc Natl Acad Sci U S A. 1987 Nov.

Abstract

Oligodeoxynucleotides lead to translation arrest of complementary mRNAs in the wheat germ translation system by a degradation of the mRNA. In an attempt to develop an effective reverse genetic approach in vivo, we demonstrate that injection of short (15- to 30-nucleotide) oligonucleotides into Xenopus oocytes leads to complete degradation of both injected and endogenous mRNAs by means of an RNase H-like activity.

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References

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