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. 2017 Sep;72(9):1415-1418.
doi: 10.1111/all.13151. Epub 2017 Apr 3.

Pru p 3, a marker allergen for lipid transfer protein sensitization also in Central Europe

N Mothes-Luksch  1 , M Raith  2 , G Stingl  3 , M Focke-Tejkl  4 , E Razzazi-Fazeli  5 , R Zieglmayer  6 , S Wöhrl  7 , I Swoboda  2
Affiliations

Pru p 3, a marker allergen for lipid transfer protein sensitization also in Central Europe

N Mothes-Luksch et al. Allergy. 2017 Sep.

Abstract

In the Mediterranean area, lipid transfer proteins (LTPs) are important causes of plant-food allergies often associated with severe allergic reactions. There, peach LTP (Pru p 3) seems to be the primary sensitizer, whereas in Central Europe, little is known about the importance of LTP sensitization. In this region, allergen extract-based diagnosis is often complicated by co-sensitization to Bet v 1, the major birch pollen allergen, its cross-reactive food allergens, and profilins. We investigated the role of LTP sensitization in Central European patients displaying strong allergic reactions to plant-derived food. Analysis of IgE reactivity revealed that ten of thirteen patients were sensitized to Pru p 3, nine to Bet v 1, and two to profilin. Our results showed that LTP sensitization represents a risk factor for severe allergic symptoms in Central Europe. Furthermore, the strong IgE reactivity detected in immunoblots of plant-food extracts indicated that Pru p 3 can be used as a marker allergen for LTP sensitization also in Central European patients.

Keywords: Pru p 3; component resolved diagnosis; food allergy; immunologic tests; lipid transfer protein.

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Figures

Figure 1
Figure 1
Representative immunoblots showing the IgE‐reactivity patterns of patients to proteins from plant extracts. Raw or cooked plant extracts were separated by 15% Tricine SDS‐PAGE, blotted onto nitrocellulose and exposed to the serum (A) of a LTP‐sensitized patient (patient 2) or (B) of a patient (patient 5) sensitized to both, LTP and Bet v 1. Molecular weight markers are shown on the left side of each blot
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