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. 2017 Mar;26(1):49-52.
doi: 10.1055/s-0036-1588062. Epub 2016 Dec 26.

Analysis of Apolipoprotein B Protein of Circulating Multiple-Modified Low-Density Lipoprotein

Emile R Zakiev  1 , Vasily N Sukhorukov  1 , Ekaterina A Ivanova  2 , Alexander N Orekhov  3
Affiliations

Analysis of Apolipoprotein B Protein of Circulating Multiple-Modified Low-Density Lipoprotein

Emile R Zakiev et al. Int J Angiol. 2017 Mar.

Abstract

Modified low-density lipoprotein (LDL) is the main source of lipid accumulation in the arterial wall affected by atherosclerosis. We aimed to compare the properties of apolipoprotein B (apoB) from native and modified LDL. Modified (desialylated) LDL and native LDL were extracted from blood of atherosclerotic patients. We characterized apoB structure of LDL particles in total LDL preparation, circulating modified LDL (cmLDL), and native LDL. Intact cmLDL had a twofold lower content of free amino groups than native LDL. Delipidated apoB from cmLDL also had a lower content of free amino groups. The rates of tryptic hydrolysis and elastase digestion of cmLDL were twofold higher in comparison to native LDL. Therefore, cmLDL from atherosclerotic patients had altered apoB properties. Our observations strengthen the hypothesis of multiple modification of LDL in the bloodstream and underscore the importance of desialylated LDL as a possible marker of atherosclerosis.

Keywords: apolipoprotein B; atherosclerosis; desialylation; low-density lipoprotein; modified LDL.

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Figures

Fig. 1
Fig. 1
Contents of free amino groups in native and circulating modified low-density lipoprotein (LDL). LDL was delipidated by treatment with chloroform–methanol mixture, and apolipoprotein B was dissolved in 0.2-M sodium hydroxide. Presented are means of three independent experiments ± standard deviation. *Significant difference from the corresponding fraction obtained from healthy donors. **Significant difference from total LDL. ***Significant difference from the corresponding fraction obtained from healthy donors, significant difference from total LDL, and significant difference from native LDL. ****Significant difference from the corresponding fraction obtained from healthy donors and significant difference from intact LDL. *****Significant difference from the corresponding fraction obtained from healthy donors, significant difference from intact LDL, significant difference from total LDL, and significant difference from native LDL. P < 0.05 (two-tailed Student's t-test).
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