Association of serum 25-hydroxyvitamin D3 with adipokines and inflammatory marker in persons with prediabetes mellitus

Abstract

Background: The association of vitamin D status and inflammation prediabetes mellitus (PreDM) individuals has not been investigated. We investigated the association of serum 25-hydroxyvitamin D₃ (25(OH) D₃) with adipokines and inflammatory markers in persons with PreDM.

Methods: In this cross-sectional study, we stratified 418 nondiabetic subjects as having PreDM or normal fasting glucose (NFG), and divided PreDM or NFG subjects into vitamin D sufficient, vitamin D insufficient and vitamin D deficient subgroups. 25(OH) D₃ concentrations were determined by HPLC. Serum tumor necrosis factor-α (TNF-α), interleiukin-6 (IL-6) and high-sensitivity C-reactive protein (hs-CRP), adiponectin, leptin and resistin were measured by ELISA.

Results: In the PreDM group, compared with those in the vitamin D sufficient subgroup, vitamin D insufficient subgroup and vitamin D deficient subgroup had higher concentrations of hs-CRP and leptin (P<0.05). In the PreDM-deficient or PreDM-insufficient subgroup, mean hs-CRP and leptin concentration was higher than in the PreDM-sufficient, NFG-deficient, NFG-insufficient and NFG-sufficient subgroups (P<0.05). Serum 25(OH) D₃ concentrations were inversely associated with hs-CRP and leptin concentrations after adjusted the BMI only in the PreDM group (P<0.05). At the multivariate analysis, hs-CRP and leptin were the major determinant of 25(OH) D₃ concentration (β=-0.174 and β=-0.171, respectively).

Conclusions: 25(OH) D₃ status in PreDM individuals was inversely correlated with concentrations of hs-CRP and leptin, suggesting their involvement in the inflammation response between vitamin D status and PreDM.

Keywords: 25-Hydroxyvitamin D(3); Adipokines; Inflammation; Prediabetes mellitus.