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. 1978 Dec;75(12):5903-7.
doi: 10.1073/pnas.75.12.5903.

Altered nucleosome spacing in newly replicated chromatin from Friend leukemia cells

R F Murphy, R B Wallace, J Bonner

Altered nucleosome spacing in newly replicated chromatin from Friend leukemia cells

R F Murphy et al. Proc Natl Acad Sci U S A. 1978 Dec.

Abstract

Chromatin from Friend leukemia cells labeled with [14C]thymidine for 24 hr followed by [3H]thymidine for 10 min is converted into nucleosomes by staphylococcal nuclease at only half the rate that total chromatin is converted. Polyacrylamide gel electrophoresis of nucleosomal DNA from cells labeled for 24 hr with [14C]thymidine followed by 10 min with [3H]thymidine demonstrates that the internucleosomal spacer of newly replicated chromatin is approximately 20 base pairs shorter than that of total chromatin. The implications of this difference for models of chromatin structure are discussed.

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References

    1. Cell. 1976 Dec;9(4 Pt 1):627-32 - PubMed
    1. Biochemistry. 1975 Aug 26;14(17):3787-94 - PubMed
    1. FEBS Lett. 1976 Oct 15;69(1):281-5 - PubMed
    1. Annu Rev Biochem. 1977;46:931-54 - PubMed
    1. Proc Natl Acad Sci U S A. 1977 Jan;74(1):79-83 - PubMed

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