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. 2016 Dec;5(4):257-261.

Impact of heat shock step on bacterial transformation efficiency

Maral Rahimzadeh  1 , Majid Sadeghizadeh  2 , Farhood Najafi  3 , Seyed Arab  4 , Hamid Mobasheri  5
Affiliations

Impact of heat shock step on bacterial transformation efficiency

Maral Rahimzadeh et al. Mol Biol Res Commun. 2016 Dec.

Abstract

CaCl2 treatment followed by heat shock is the most common method for artificial transformation. Here, the cells were transformed using CaCl2 treatment either with heat shock (standard protocol) or without heat shock (lab protocol) to comprehend the difference in transformation efficiency. The BL21 strain of Escherichia coli (E. coli) was being susceptible using CaCl2 treatment. Some Cells were kept at -80 oC while the others were kept at 4 ˚C. Afterwards the susceptible cells were transformed using either standard or lab protocol. The transformation efficiency between cells experienced heat shock and those were not influenced by heat shock was almost the same. Moreover, regardless of transformation protocol, the cells kept at 4 ˚C were transformed more efficiently in compared to those were kept at -80 oC.

Keywords: Artificial transformation; E. coli; Heat shock; Transformation efficiency.

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Figures

Figure 1
Figure 1
Transformation efficiencies of various conditions
Figure 2
Figure 2
Successful diluted bacterial transformation plates using standard and lab protocol (The samples were diluted 100 times
See this image and copyright information in PMC

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References

    1. Chen I, Dubnau D. DNA uptake during bacterial transformation. Nat Rev Microbiol. 2004;2:241–249. - PubMed
    1. Panja S, Saha S, Jana B, Basu T. Role of membrane potential on artificial transformation of E coli with plasmid DNA. J Biotechnol. 2006;127:14–20. - PubMed
    1. Divya Prakash G, Anish RV, Jagadeesh G, Chakravortty D. Bacterial transformation using micro-shock waves. Anal Biochem. 2011;419:292–301. - PubMed
    1. Chung CT, Niemela SL, Miller RH. One-step preparation of competent Escherichia coli: transformation and storage of bacterial cells in the same solution. Proc Natl Acad Sci USA. 1989;86:2172–2175. - PMC - PubMed
    1. Inoue H, Nojima H, Okayama H. High efficiency transformation of Escherichia coli with plasmids. Gene. 1990;96:23–28. - PubMed

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