Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2017 Feb 28;22(3):374.
doi: 10.3390/molecules22030374.

Influence of Indole-3-Acetic Acid and Gibberellic Acid on Phenylpropanoid Accumulation in Common Buckwheat (Fagopyrum esculentum Moench) Sprouts

Chang Ha Park  1 , Hyeon Ji Yeo  2 , Yun Ji Park  3 , Abubaker M A Morgan  4 , Mariadhas Valan Arasu  5 , Naif Abdullah Al-Dhabi  6 , Sang Un Park  7
Affiliations

Influence of Indole-3-Acetic Acid and Gibberellic Acid on Phenylpropanoid Accumulation in Common Buckwheat (Fagopyrum esculentum Moench) Sprouts

Chang Ha Park et al. Molecules. .

Abstract

We investigated the effects of natural plant hormones, indole-3-acetic (IAA) acid and gibberellic acid (GA), on the growth parameters and production of flavonoids and other phenolic compounds in common buckwheat sprouts. A total of 17 phenolic compounds were identified using liquid chromatography-mass spectrometry (LC-MS) analysis. Among these, seven compounds (4-hydroxybenzoic acid, catechin, chlorogenic acid, caffeic acid, epicatechin, rutin, and quercetin) were quantified by high-performance liquid chromatography (HPLC) after treating the common buckwheat sprouts with different concentrations of the hormones IAA and GA. At a concentration of 0.5 mg/L, both IAA and GA exhibited the highest levels of growth parameters (shoot length, root length, and fresh weight). The HPLC analysis showed that the treatment of sprouts with IAA at concentrations ranging from 0.1 to 1.0 mg/L produced higher or comparable levels of the total phenolic compounds than the control sprout and enhanced the production of rutin. Similarly, the supplementation with 0.1 and 0.5 mg/L GA increased the content of rutin in buckwheat sprouts. Our results suggested that the treatment with optimal concentrations of IAA and GA enhanced the growth parameters and accumulation of flavonoids and other phenolic compounds in buckwheat sprouts.

Keywords: Fagopyrum esculentum Moench; common buckwheat; gibberellic acid; indole-3-acetic acid; phenylpropanoid.

PubMed Disclaimer

Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Effect of indole-3-acetic (IAA) and gibberellic acid (GA) on sprout growth.
See this image and copyright information in PMC

References

    1. Jeon Y.-J., Kang E.-S., Hong K.-W. A PCR method for rapid detection of buckwheat ingredients in food. J. Korean Soc. Appl. Biol. Chem. 2007;50:276–280.
    1. Golisz A., Lata B., Gawronski S.W., Fujii Y. Specific and total activities of the allelochemicals identified in buckwheat. Weed Biol. Manag. 2007;7:164–171. doi: 10.1111/j.1445-6664.2007.00252.x. - DOI
    1. Kim D.W., Hwang I.K., Lim S.S., Yoo K.Y., Li H., Kim Y.S., Kwon D.Y., Moon W.K., Kim D.W., Won M.H. Germinated buckwheat extract decreases blood pressure and nitrotyrosine immunoreactivity in aortic endothelial cells in spontaneously hypertensive rats. Phytother. Res. 2009;23:993–998. doi: 10.1002/ptr.2739. - DOI - PubMed
    1. Kim C.D., Lee W.-K., No K.-O., Park S.-K., Lee M.-H., Lim S.R., Roh S.-S. Anti-allergic action of buckwheat (Fagopyrum esculentum Moench) grain extract. Int. Immunopharmacol. 2003;3:129–136. doi: 10.1016/S1567-5769(02)00261-8. - DOI - PubMed
    1. Amarowicz R., Dykes G.A., Pegg R.B. Antibacterial activity of tannin constituents from Phaseolus vulgaris, Fagoypyrum esculentum, Corylus avellana and Juglans nigra. Fitoterapia. 2008;79:217–219. doi: 10.1016/j.fitote.2007.11.019. - DOI - PubMed

MeSH terms

LinkOut - more resources