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. 2017 May-Jun;21(3):282-289.
doi: 10.1016/j.bjid.2017.01.004. Epub 2017 Mar 6.

Virulence genes and genetic relationship of L. monocytogenes isolated from human and food sources in Brazil

Rosana Macedo de Almeida  1 , André Victor Barbosa  2 , Rodrigo de Castro Lisbôa  3 , André Felipe das Mercês Santos  4 , Ernesto Hofer  3 , Deyse Christina Vallim  3 , Cristina Barroso Hofer  5
Affiliations

Virulence genes and genetic relationship of L. monocytogenes isolated from human and food sources in Brazil

Rosana Macedo de Almeida et al. Braz J Infect Dis. 2017 May-Jun.

Abstract

The herein presented assay provided a bacteriological and molecular characterization of 100 samples of L. monocytogenes isolated from human (43) and food (57) sources, from several regions of Brazil, and collected between 1975 and 2013. Antigenic characterization defined 49% of serotype 4b samples, followed by 28% of serotype 1/2b, 14% of serotype 1/2c, 8% of serotype 1/2a, and 1% of serotype 3b. Both type of samples from human and food origin express the same serotype distribution. Multiplex PCR analysis showed 13 strains of type 4b with the amplification profile 4b-VI (Variant I). Virulence genes hly, inlA, inlB, inlC, inlJ, actA, plcA, and prfA were detected in all samples, highlighting a deletion of 105pb on the actA gene in 23% of serotype 4b samples. Macrorestriction profile with ApaI at PFGE showed 55 pulsotypes, with the occurrence of the same pulsotype in hospitalized patients in São Paulo in 1992 and 1997, and two other highly related pulsotypes in patients hospitalized in Rio de Janeiro in 2008. Recognized pulsotypes in listeriosis cases have also been detected in food. Thus, the prevalence of a serotype and the persistence of certain pulsotypes herald future problems.

Keywords: Brazil; Food; Listeria monocytogenes; Listeriosis; PFGE; Virulence.

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Figures

Fig. 1
Fig. 1
Dendrogram containing the distribution of serotypes 1/2b and 4b using the PFGE technique and analyzed using the Dice coefficient with 1.5% tolerance, using UPGMA.
Fig. 2
Fig. 2
Dendrogram containing the distribution of serotypes 1/2a and 1/2c using the PFGE technique and analyzed using the Dice coefficient with 1.5% tolerance, using UPGMA. *State – Distrito Federal (DF), Paraíba (PB), Pernambuco (PE), Rio de Janeiro (RJ), São Paulo (SP), Paraná (PR), Rio Grande do Sul (RS), Santa Catarina (SC).
Fig. 3
Fig. 3
Isolation frequency of L. monocytogenes pulsotypes.
Fig. 4
Fig. 4
Isolation frequency of recurring L. monocytogenes pulsotypes in different years.
See this image and copyright information in PMC

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