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. 2017 May;55(5):1585-1587.
doi: 10.1128/JCM.00126-17. Epub 2017 Mar 8.

Evaluation of an Alternative Recombinant Thermostable Thermus thermophilus (r Tth)-Based Real-Time Reverse Transcription-PCR Kit for Detection of Rotavirus A

Eric M Katz  1 , Rashi Gautam  1 , Michael D Bowen  2
Affiliations

Evaluation of an Alternative Recombinant Thermostable Thermus thermophilus (r Tth)-Based Real-Time Reverse Transcription-PCR Kit for Detection of Rotavirus A

Eric M Katz et al. J Clin Microbiol. 2017 May.
No abstract available

Keywords: qRT-PCR; rTth; rotavirus.

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Figures

FIG 1
FIG 1
Amplification curves of 10-fold dilutions of the RVA strain Wa dsRNA transcript spiked with MS2 bacteriophage RNA (from 6.5 × 106 to 6.5 copies per reaction), obtained with the EMD Millipore NSP3 qRT-PCR assay. Using the threshold for delta Rn (the normalized reporter value [Rn] of the reaction minus the Rn of the baseline signal) (green line), black curves show the 10-fold dilutions of the NSP3 gene transcript and red curves show MS2 IPC amplification. The graph showing the CT value versus the log copy number was fitted with a regression line, and the slope for calculation of efficiency was obtained from the regression line. The fluorescent signals from RVA-negative samples and no-template controls are indicated by the blue outline.
See this image and copyright information in PMC

References

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    1. Gautam R, Mijatovic-Rustempasic S, Esona MD, Tam KI, Osbourne Q, Bowen MD. 2016. One-step multiplex real-time RT-PCR assay for detecting and genotyping wild-type group A rotavirus strains and vaccine strains (Rotarix® and RotaTeq®) in stool samples. PeerJ 4:e1560. doi:10.7717/peerj.1560. - DOI - PMC - PubMed
    1. Freeman MM, Kerin TK, Hull J, McCaustland K, Gentsch J. 2008. Enhancement of detection and quantification of rotavirus in stool using a modified real-time RT-PCR assay. J Med Virol 80:1489–1496. doi:10.1002/jmv.21228. - DOI - PubMed
    1. National Committee for Clinical Laboratory Standards. 2002. User protocol for evaluation of qualitative test performance; approved guideline. NCCLS document EP12-A. Clinical and Laboratory Standards Institute, Wayne, PA.

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