Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1987;97(3-4):365-72.
doi: 10.1007/BF01314434.

Expression of an early Epstein-Barr virus antigen (EA-D) in E. coli. Brief report

D Roeckel  1 , H Boos, N Mueller-Lantzsch
Affiliations

Expression of an early Epstein-Barr virus antigen (EA-D) in E. coli. Brief report

D Roeckel et al. Arch Virol. 1987.

Abstract

The 1.34 kb BcII-BgIII-fragment of the BamHI-M region of Epstein-Barr virus genome, comprising the complete BMRF1 open reading frame, was cloned into the tryptophan regulated E. coli expression vector pATH1. The resulting fusion protein, having a molecular weight of 80 kd, is recognized not only by anti-early antigen (EA)-positive human sera but also by the monoclonal antibody R3 directed against the diffuse component of EA (EA-D). A possible use for this fusion protein as an indicator protein in diagnosis of IgA antibodies against EA-D is presented.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

References

    1. J Immunol Methods. 1983 Oct 14;63(2):171-85 - PubMed
    1. Proc Natl Acad Sci U S A. 1984 Jul;81(14):4568-72 - PubMed
    1. Virology. 1984 Aug;137(1):9-19 - PubMed
    1. J Virol. 1986 Oct;60(1):149-56 - PubMed
    1. J Bacteriol. 1966 Mar;91(3):1248-56 - PubMed

Publication types

Substances

LinkOut - more resources