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. 2017 Mar 10;355(6329):eaaf4704.
doi: 10.1126/science.aaf4704.

"Perfect" designer chromosome V and behavior of a ring derivative

Ze-Xiong Xie  1   2 , Bing-Zhi Li  1   2 , Leslie A Mitchell  3 , Yi Wu  1   2 , Xin Qi  1   2 , Zhu Jin  1   2 , Bin Jia  1   2 , Xia Wang  1   2 , Bo-Xuan Zeng  1   2 , Hui-Min Liu  1   2 , Xiao-Le Wu  1   2 , Qi Feng  1   2 , Wen-Zheng Zhang  1   2 , Wei Liu  1   2 , Ming-Zhu Ding  1   2 , Xia Li  1   2 , Guang-Rong Zhao  1   2 , Jian-Jun Qiao  1   2 , Jing-Sheng Cheng  1   2 , Meng Zhao  1   2 , Zheng Kuang  3 , Xuya Wang  3 , J Andrew Martin  3 , Giovanni Stracquadanio  4   5 , Kun Yang  4 , Xue Bai  1   2 , Juan Zhao  1   2 , Meng-Long Hu  1   2 , Qiu-Hui Lin  1   2 , Wen-Qian Zhang  1   2 , Ming-Hua Shen  1   2 , Si Chen  1   2 , Wan Su  1   2 , En-Xu Wang  1   2 , Rui Guo  1   2 , Fang Zhai  1   2 , Xue-Jiao Guo  1   2 , Hao-Xing Du  1   2 , Jia-Qing Zhu  1   2 , Tian-Qing Song  1   2 , Jun-Jun Dai  1   2 , Fei-Fei Li  1   2 , Guo-Zhen Jiang  1   2 , Shi-Lei Han  1   2 , Shi-Yang Liu  1   2 , Zhi-Chao Yu  1   2 , Xiao-Na Yang  1   2 , Ken Chen  1   2 , Cheng Hu  1   2 , Da-Shuai Li  1   2 , Nan Jia  1   2 , Yue Liu  1   2 , Lin-Ting Wang  1   2 , Su Wang  1   2 , Xiao-Tong Wei  1   2 , Mei-Qing Fu  1   2 , Lan-Meng Qu  1   2 , Si-Yu Xin  1   2 , Ting Liu  1   2 , Kai-Ren Tian  1   2 , Xue-Nan Li  1   2 , Jin-Hua Zhang  1   2 , Li-Xiang Song  1   2 , Jin-Gui Liu  1   2 , Jia-Fei Lv  1   2 , Hang Xu  1   2 , Ran Tao  1   2 , Yan Wang  1   2 , Ting-Ting Zhang  1   2 , Ye-Xuan Deng  1   2 , Yi-Ran Wang  1   2 , Ting Li  1   2 , Guang-Xin Ye  1   2 , Xiao-Ran Xu  1   2 , Zheng-Bao Xia  1   2 , Wei Zhang  1   2 , Shi-Lan Yang  1   2 , Yi-Lin Liu  1   2 , Wen-Qi Ding  1   2 , Zhen-Ning Liu  1   2 , Jun-Qi Zhu  1   2 , Ning-Zhi Liu  1   2 , Roy Walker  6 , Yisha Luo  6 , Yun Wang  7 , Yue Shen  7 , Huanming Yang  7   8 , Yizhi Cai  6 , Ping-Sheng Ma  1 , Chun-Ting Zhang  1 , Joel S Bader  4 , Jef D Boeke  3 , Ying-Jin Yuan  9   2
Affiliations

"Perfect" designer chromosome V and behavior of a ring derivative

Ze-Xiong Xie et al. Science. .

Abstract

Perfect matching of an assembled physical sequence to a specified designed sequence is crucial to verify design principles in genome synthesis. We designed and de novo synthesized 536,024-base pair chromosome synV in the "Build-A-Genome China" course. We corrected an initial isolate of synV to perfectly match the designed sequence using integrative cotransformation and clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9)-mediated editing in 22 steps; synV strains exhibit high fitness under a variety of culture conditions, compared with that of wild-type V strains. A ring synV derivative was constructed, which is fully functional in Saccharomyces cerevisiae under all conditions tested and exhibits lower spore viability during meiosis. Ring synV chromosome can extends Sc2.0 design principles and provides a model with which to study genomic rearrangement, ring chromosome evolution, and human ring chromosome disorders.

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