Design of a synthetic yeast genome

Sarah M Richardson^{1

2}, Leslie A Mitchell^{2

3}, Giovanni Stracquadanio^{1

2

4}, Kun Yang^{1

2}, Jessica S Dymond², James E DiCarlo², Dongwon Lee¹, Cheng Lai Victor Huang², Srinivasan Chandrasegaran⁵, Yizhi Cai^{2

6}, Jef D Boeke^{7

3}, Joel S Bader^{8

2}

Affiliations

¹ Department of Biomedical Engineering, Johns Hopkins University, Baltimore, MD 21205, USA.
² High Throughput Biology Center, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
³ Institute for Systems Genetics and Department of Biochemistry and Molecular Pharmacology, New York University Langone Medical Center, New York, NY 10016, USA.
⁴ School of Computer Science and Electronic Engineering, University of Essex, Wivenhoe Park, Colchester CO4 3SQ, UK.
⁵ Department of Environmental Health Science, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, MD 21205, USA.
⁶ University of Edinburgh, School of Biological Sciences, University of Edinburgh, Edinburgh EH9 3BF, UK.
⁷ High Throughput Biology Center, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA. jef.boeke@nyumc.org joel.bader@jhu.edu.
⁸ Department of Biomedical Engineering, Johns Hopkins University, Baltimore, MD 21205, USA. jef.boeke@nyumc.org joel.bader@jhu.edu.

PMID: 28280199
DOI: 10.1126/science.aaf4557

Design of a synthetic yeast genome

Sarah M Richardson et al. Science. 2017.

. 2017 Mar 10;355(6329):1040-1044.

doi: 10.1126/science.aaf4557.

Authors

Affiliations

¹ Department of Biomedical Engineering, Johns Hopkins University, Baltimore, MD 21205, USA.
² High Throughput Biology Center, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
³ Institute for Systems Genetics and Department of Biochemistry and Molecular Pharmacology, New York University Langone Medical Center, New York, NY 10016, USA.
⁴ School of Computer Science and Electronic Engineering, University of Essex, Wivenhoe Park, Colchester CO4 3SQ, UK.
⁵ Department of Environmental Health Science, Bloomberg School of Public Health, Johns Hopkins University, Baltimore, MD 21205, USA.
⁶ University of Edinburgh, School of Biological Sciences, University of Edinburgh, Edinburgh EH9 3BF, UK.
⁷ High Throughput Biology Center, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA. jef.boeke@nyumc.org joel.bader@jhu.edu.
⁸ Department of Biomedical Engineering, Johns Hopkins University, Baltimore, MD 21205, USA. jef.boeke@nyumc.org joel.bader@jhu.edu.

PMID: 28280199
DOI: 10.1126/science.aaf4557

Abstract

We describe complete design of a synthetic eukaryotic genome, Sc2.0, a highly modified Saccharomyces cerevisiae genome reduced in size by nearly 8%, with 1.1 megabases of the synthetic genome deleted, inserted, or altered. Sc2.0 chromosome design was implemented with BioStudio, an open-source framework developed for eukaryotic genome design, which coordinates design modifications from nucleotide to genome scales and enforces version control to systematically track edits. To achieve complete Sc2.0 genome synthesis, individual synthetic chromosomes built by Sc2.0 Consortium teams around the world will be consolidated into a single strain by "endoreduplication intercross." Chemically synthesized genomes like Sc2.0 are fully customizable and allow experimentalists to ask otherwise intractable questions about chromosome structure, function, and evolution with a bottom-up design strategy.

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Comment in

Synthetic yeast chromosomes help probe mysteries of evolution.
Maxmen A. Maxmen A. Nature. 2017 Mar 9;543(7645):298-299. doi: 10.1038/nature.2017.21615. Nature. 2017. PMID: 28300123 No abstract available.
How dare you call us diplomats.
Moro-Martín A. Moro-Martín A. Nature. 2017 Mar 14;543(7645):289. doi: 10.1038/543289a. Nature. 2017. PMID: 28300124 No abstract available.
Synthetic Biology: Building a custom eukaryotic genome de novo.
Burgess DJ. Burgess DJ. Nat Rev Genet. 2017 Apr 12;18(5):274. doi: 10.1038/nrg.2017.30. Nat Rev Genet. 2017. PMID: 28400600 No abstract available.
A spring collection of designer yeast chromosomes.
Marcinkiewicz K. Marcinkiewicz K. Nat Biotechnol. 2017 Jun 7;35(6):522. doi: 10.1038/nbt.3890. Nat Biotechnol. 2017. PMID: 28591121 No abstract available.

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Design of a synthetic yeast genome

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Design of a synthetic yeast genome

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Full Text Sources

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