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. 2017 Feb 14;9(Suppl 1/2016 to N 4/2016):10-20.
doi: 10.11138/orl/2016.9.1S.010. eCollection 2016 Jan-Mar.

Implant Dentistry: Monitoring of Bacteria Along the Transmucosal Passage of the Healing Screw in Absence of Functional Load

F Meynardi  1 , M E Pasqualini  2 , F Rossi  3 , L Dal Carlo  4 , M Nardone  5 , L Baggi  6
Affiliations

Implant Dentistry: Monitoring of Bacteria Along the Transmucosal Passage of the Healing Screw in Absence of Functional Load

F Meynardi et al. Oral Implantol (Rome). .

Abstract

Purpose: To assess the changes in bacterial profile along the transmucosal path of healing screws placed immediately after insertion of two-piece endosseus implants during the 4-month osseointegration phase, in absence of functional load.

Materials and methods: Two site-specific samples were collected at the peri-implant mucosa of the healing screws of 80 two-piece implants, for a total of 640 samples. Implants placement was performed following a single protocol with flapless technique, in order to limit bacterial contamination of the surgical site. Identical healing screws (5 mm diameter/4 mm height) were used for each of the 80 implants. During the 4 months of the study, the patients followed a standard oral care regimen with no special hygiene maneuvers at the collection sites.

Results: The present research documents that during the 4-month period prior to application of function load the bacterial profile of all sites exhibited a clear prevalence of cocci at the interface between implant neck and osteoalveolar crest margin.

Conclusions: A potentially pathogenic bacterial flora developed only along the peri-implant transmucosal path.

Keywords: absence of functional load; bacterial morphological profile; healing screw; transmucosal peri-implant path; two-piece implant.

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Figures

Figure 1
Figure 1
Healing titanium screw of one of the 80 cases analyzed.
Figure 2
Figure 2
The first phase of withdrawal was performed using a sterile tip in scope of the transmucosal route (TMP), indicated by the arrows, while maintaining the healing screw in situ with the presence of its bacterial film (plaque) does not vary the conditions for microbial between the various levels of the levy niche.
Figure 3
Figure 3
Second phase of withdrawal (CMI): blue arrows show the margin-alveolar crestal bone after the healing screw removal. Observe the amount of plaque.
Figure 4
Figure 4
The arrows indicate the sampling sites. The transmucosal route TMP (yellow arrows) and the margin alveolar crestal bone-CMI (blue arrows).
Figure 5
Figure 5
Position provides amply TMP (yellow arrows) and marginalveolar crestal bone IMC (blue arrows) seen in the image Xray.
Figure 6
Figure 6
The plaque adherent to the surface of the titanium healing screw.
Figure 7
Figure 7
40x microscope view of the levy of TMP provides amply when you notice the presence of all bacterial morphology (cocci, rods, filaments and convoluted).
Graphic 1
Graphic 1
One-month follow-up. The differences in bacterial loading in CMI and TMP are statistical significant by using Mann-Whitney U test.
Graphic 2
Graphic 2
Two-month follow-up. The differences in bacterial loading in CMI and TMP are statistical significant by using Mann-Whitney U test.
Graphic 3
Graphic 3
Three-month follow-up. The differences in bacterial loading in CMI and TMP are statistical significant by using Mann-Whitney U test.
Graphic 4
Graphic 4
Fou-month follow-up. The differences in bacterial loading in CMI and TMP are statistical significant by using Mann-Whitney U test.
See this image and copyright information in PMC

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References

    1. Listgarten MA. The structure of dental plaque. Periodontol. 2000;1994(5):52–65. - PubMed
    1. Lindhe J, Nyman S. Textbook of clinical periodontology. Place: Munksgaard; 1989.
    1. Meynardi F, Pasqualini ME, Rossi F, Biancotti P. Confronto del profilo batterico presente in siti implantari con mesostruttura solidarizzata mediante barra saldata e con perno moncone singolo. Doctor Os. 2013;5:411–15.
    1. Listgarten MA. The role of dental plaque in gingivitis and periodontitis. J Clin Periodontol. 1988;15:485–7. - PubMed
    1. Gandolfo S, Meynardi F, Corrente G, Nelken A. Analisi microbiologica a fresco della placca dento-gengivale. RIS. 1994:275–86.

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