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. 1987;58(2-3):243-56.
doi: 10.1016/0378-1119(87)90379-9.

E1a inducibility of the adenoviral early E2a promoter is determined by specific combinations of sequence elements

D A Zajchowski  1 , H Boeuf, C Kédinger
Affiliations

E1a inducibility of the adenoviral early E2a promoter is determined by specific combinations of sequence elements

D A Zajchowski et al. Gene. 1987.

Abstract

By transient expression analysis in HeLa cells of adenovirus-2 E2a early (E2aE) promoter mutants and hybrid E2aE-beta-globin gene constructs, we demonstrate the existence of three nucleotide (nt) sequence elements involved in the E1a-responsiveness of the E2aE transcription unit: element I, localized within a segment (nt -13 to +62) surrounding the major E2aE cap site (nt + 1); element II (between nt -71 and -29), and element III (between nt -146 and -86). Each element is unable by itself to confer E1a responsiveness. Only combinations of sequences including elements I and II (spanning nt -71 and +62) or II and III (spanning nt -146 and -29) ensure maximal inducibility, for which element II appears of central importance.

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