doi: 10.1016/0378-1119(87)90384-2.
Convenient vectors for cloning and sequencing EcoRI and HindIII fragments
Affiliations
- PMID: 2828191
- DOI: 10.1016/0378-1119(87)90384-2
Item in Clipboard
Convenient vectors for cloning and sequencing EcoRI and HindIII fragments
Gene.
1987.
Abstract
The polylinker regions of plasmid pUC and bacteriophage M13mp vectors have been specifically modified to provide alternative positions for cloning and reexcising EcoRI and HindIII fragments; the EcoRI and HindIII sites have been moved internal to BamHI and Bg/II sites. The location of EcoRI and HindIII sites in these HinEco vectors allows either selective linearization or excision of the cloned fragments at unique flanking sites.
Similar articles
-
A new selective phage cloning vector, lambda 2001, with sites for XbaI, BamHI, HindIII, EcoRI, SstI and XhoI.Gene. 1984 Dec;32(1-2):217-24. doi: 10.1016/0378-1119(84)90049-0. Gene. 1984. PMID: 6099311
-
Cloned fragments of human adenovirus type-12 DNA.Gene. 1981 Nov;15(2-3):273-8. doi: 10.1016/0378-1119(81)90136-0. Gene. 1981. PMID: 6271646
-
Molecular cloning and restriction endonuclease mapping of the murine cytomegalovirus genome (Smith Strain).Virology. 1983 Aug;129(1):94-106. doi: 10.1016/0042-6822(83)90398-7. Virology. 1983. PMID: 6310888
-
Modification of the bacteriophage vector M13mp2: introduction of new restriction sites for cloning.Gene. 1981 Nov;15(2-3):167-76. doi: 10.1016/0378-1119(81)90126-8. Gene. 1981. PMID: 6271640
-
Gene cloning: studies of nutritional regulation of gene expression.Proc Nutr Soc. 1996 Mar;55(1B):575-81. doi: 10.1079/pns19960050. Proc Nutr Soc. 1996. PMID: 8832820 Review. No abstract available.
Cited by
-
Methionine-141 directly influences the binding of 4-methylpyrazole in human sigma sigma alcohol dehydrogenase.Protein Sci. 1999 Dec;8(12):2639-44. doi: 10.1110/ps.8.12.2639. Protein Sci. 1999. PMID: 10631979 Free PMC article.
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
