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Randomized Controlled Trial
. 2017 Mar 11:23:1242-1246.
doi: 10.12659/msm.898589.

Detection of Cytomegalovirus (CMV) Infection in Wheezing Infants by Urine DNA and Serum IgG Testing

Zhao-Cheng Zeng  1 , Qing Chang  2 , Zhi-Wei Sun  1 , Ming-Mei Song  1 , Xin-Ling Jin  1 , Shu-Ya Jiang  1 , Xia Yang  1
Affiliations
Randomized Controlled Trial

Detection of Cytomegalovirus (CMV) Infection in Wheezing Infants by Urine DNA and Serum IgG Testing

Zhao-Cheng Zeng et al. Med Sci Monit. .

Abstract

BACKGROUND The aim of this study was to investigate the involvement of CMV infection in wheezing infants and the association between CMV-DNA and immunoglobulins (Igs). MATERIAL AND METHODS A total of 243 wheezing infants and 3,000 parturients were enrolled in this study. The infants were randomly grouped to receive blood HCMV-DNA tests (n=46) or urine HCMV-DNA tests (n=197). Furthermore, all participants had serum CMV-specific IgM and IgG testing. Afterwards, 10 HCMV-IgG positive infants were randomly selected for simultaneous blood and urine HCMV-DNA tests, and 25 HCMV-IgG positive puerperants were randomly selected for urine HCMV-DNA tests. RESULTS The detection rate of urine HCMV-DNA was significantly higher than that of blood HCMV-DNA (67.5% vs. 13.0%, p<0.001). Fifteen (6.2%) and 190 (80.0%) infants showed positive CMV-specific IgM and IgG results (p<0.001), respectively. Among the 10 HCMV-IgG positive infants tested further, only two infants had positive HCMV-DNA blood tests, while all of the 10 infants had positive HCMV-DNA urine tests. However, HCMV-DNA was not detected in the urine of the 25 randomly selected parturients positive for HCMV-IgG. CONCLUSIONS CMV infection may be one of the causes of wheezing in infants; CMV infection can be detected by urine-HCMV-DNA and serum HCMV-IgG testing. Infants were more susceptible to CMV infection than parturients.

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Conflict of interest statement

Conflicts of interest

None.

Figures

Figure 1
Figure 1
Study flow. (A) The enrolled 243 infants were randomly grouped for blood (n=46) and urine (n=197) HCMV-DNA tests. (B) All enrolled 243 infants received serum HCMV-IgM and HCMV-IgG tests. Afterwards, 10 HCMV-IgG positive infants were randomly selected for blood and urine HCMV-DNA tests. (C) A total of 3,000 parturients were tested for serum HCMV-IgM and HCMV-IgG. Afterwards, 25 HCMV-IgG positive parturients were randomly selected for urine HCMV-DNA test.
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References

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