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. 2017 Jun:138:163-169.
doi: 10.1016/j.phytochem.2017.02.029. Epub 2017 Mar 8.

Isolation and structure elucidation of cyclopeptide alkaloids from Ziziphus nummularia and Ziziphus spina-christi by HPLC-DAD-MS and HPLC-PDA-(HRMS)-SPE-NMR

Emmy Tuenter  1 , Kenn Foubert  2 , Dan Staerk  3 , Sandra Apers  2 , Luc Pieters  2
Affiliations

Isolation and structure elucidation of cyclopeptide alkaloids from Ziziphus nummularia and Ziziphus spina-christi by HPLC-DAD-MS and HPLC-PDA-(HRMS)-SPE-NMR

Emmy Tuenter et al. Phytochemistry. 2017 Jun.

Abstract

Seven cyclopeptide alkaloids were isolated from the stem bark of Ziziphus nummularia and Ziziphus spina-christi. Three previously undescribed compounds were identified: nummularine-U, spinanine-B and spinanine-C, together with the known compounds mauritine-F, nummularine-D, nummularine-E and amphibine-D. For their purification either semi-preparative HPLC with DAD and ESIMS detection or HPLC-PDA-(HRMS)-SPE-NMR was applied, together with conventional separation methods. Their structures were elucidated by spectroscopic means.

Keywords: Cyclopeptide alkaloids; Rhamnaceae; Ziziphus nummularia; Ziziphus spina-christi.

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