Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1987 Nov;7(11):3923-8.
doi: 10.1128/mcb.7.11.3923-3928.1987.

Expression of HLA-DR antigen in human class II mutant B-cell lines by double infection with retrovirus vectors

Z Yang  1 , A J Korman, J Cooper, D Pious, R S Accolla, R C Mulligan, J L Strominger
Affiliations

Expression of HLA-DR antigen in human class II mutant B-cell lines by double infection with retrovirus vectors

Z Yang et al. Mol Cell Biol. 1987 Nov.

Abstract

A new retrovirus vector containing the gene for hygromycin B resistance (hyg) as a selectable marker under the control of an internal simian virus 40 promoter was constructed. It was used, together with an analogous previously described vector, DO1, which contains the gene for G418 resistance, to introduce and express the genes for the two chains of a human class II major histocompatibility complex antigen in NIH 3T3 cells. In addition, these vectors were used to express DR antigens in two human mutant B-lymphoblastoid cell lines, one of which was deleted for both alleles of the DR alpha gene and the other of which expressed no class II antigens because of a genetic defect in a putative trans-acting regulatory factor.

PubMed Disclaimer

References

    1. Cell. 1983 May;33(1):153-9 - PubMed
    1. Gene. 1983 Nov;25(2-3):179-88 - PubMed
    1. Proc Natl Acad Sci U S A. 1984 Dec;81(23):7549-53 - PubMed
    1. Mol Cell Biol. 1985 Feb;5(2):410-3 - PubMed
    1. Science. 1985 May 3;228(4699):554-8 - PubMed

Publication types