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. 2017 Jan;6(1):26-32.

Effect of Basic Fibroblast Growth Factor on Achilles Tendon Healing in Rabbit

Arash Najafbeygi  1 , Mohammad Javad Fatemi  1 , Amir Hussein Lebaschi  1 , Seyed Jaber Mousavi  1 , Seyed Abouzar Husseini  1 , Mitra Niazi  1
Affiliations

Effect of Basic Fibroblast Growth Factor on Achilles Tendon Healing in Rabbit

Arash Najafbeygi et al. World J Plast Surg. 2017 Jan.

Abstract

Background: Tendon injuries are common and it takes a long time for an injured tendon to heal. Adverse phenomena such as adhesion and rupture are associated with these injuries. Finding a method to reduce the time required for healing which improves the final outcome, will lead to decreased frequency and intensity of adverse consequences. This study was designed to investigate the effects of basic fibroblast growth factor on the healing of the Achilles tendon in rabbits.

Methods: In 10 New Zealand white rabbits, Achilles tendon was cut at the intersection of the distal and middle thirds on both hind legs. One microgram of recombinant basic fibroblast growth factor (bFGF) was injected in the proximal and distal stumps of the cut tendon on the right side (study group). Normal saline of equal volume was injected on the left side in the same way (control group). Then the tendons were repaired with 5/0 nylon using modified Kessler technique. A cast was made to immobilize each leg. On day 42, rabbits were euthanized and both hind legs were amputated. Tensometry and histopathologic examination were done on specimens.

Results: In tensometric studies, more force was required to rupture the repair site in study group. In histopathologic examination, collagen fibers had significantly better orientation and organization in the study group. No difference was noted regarding number of fibroblast and fibrocytes, and degree of angiogenesis in the two groups.

Conclusion: Application of basic fibroblast growth factor at tendon repair site improves the healing process through improvement of collagen fiber orientation and increase in biomechanical resistance.

Keywords: Fibroblast growth factor; Healing; Rabbit; Tendon injury.

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Figures

Fig. 1
Fig. 1
Fourth rabbit right and left tendon Tensiometrical digram
Fig. 2
Fig. 2
Histopathologic findings: number of fibroblasts and fibrocytes as well as Angiogenesis appeared better in the study group, but this difference was not statistically significant. Collagen orientation was moderate in all cases of the study group and a significant difference compared to the control group
See this image and copyright information in PMC

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