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. 2017 Feb;41(1):113-120.
doi: 10.5535/arm.2017.41.1.113. Epub 2017 Feb 28.

Effect of Intravenous Infusion of G-CSF-Mobilized Peripheral Blood Mononuclear Cells on Upper Extremity Function in Cerebral Palsy Children

Kyeong Il Park  1 , Young-Ho Lee  2 , Wee-Jin Rah  2 , Seung Hwi Jo  1 , Si-Bog Park  1 , Seung Hoon Han  1 , Hani Koh  3 , Jin Young Suh  4 , Jang Soo Um  1 , Eun Hye Choi  1 , Un Jin Park  1 , Mi Jung Kim  1
Affiliations

Effect of Intravenous Infusion of G-CSF-Mobilized Peripheral Blood Mononuclear Cells on Upper Extremity Function in Cerebral Palsy Children

Kyeong Il Park et al. Ann Rehabil Med. 2017 Feb.

Abstract

Objective: To investigate the effect of intravenous infusion of peripheral blood mononuclear cells (mPBMC) mobilized by granulocyte-colony stimulating factor (G-CSF) on upper extremity function in children with cerebral palsy (CP).

Methods: Fifty-seven children with CP were enrolled. Ten patients were excluded due to follow-up loss. In total, 47 patients (30 males and 17 females) were analyzed. All patients' parents provided signed consent before the start of the study. After administration of G-CSF for 5 days, mPBMC was collected and cryopreserved. Patients were randomized into two groups 1 month later. Twenty-two patients were administered mPBMC and 25 patients received normal saline as placebo. Six months later, the two groups were switched, and administered mPBMC and placebo, respectively. Quality of Upper Extremity Skills Test (QUEST) and the Manual Ability Classification System (MACS) were used to evaluate upper motor function.

Results: All subdomain and total scores of QUEST were significantly improved after mPBMC and placebo infusion, without significant differences between mPBMC and placebo groups. A month after G-CSF, all subdomain and total scores of QUEST were improved. The level of MACS remained unchanged in both mPBMC and placebo groups.

Conclusion: In this study, intravenously infused mPBMC showed no significant effect on upper extremity function in children with CP, as compared to placebo. The effect of mPBMC was likely masked by the effect of G-CSF, which was used in both groups and/or G-CSF itself might have other neurotrophic potentials in children with CP.

Keywords: Cerebral palsy; Granulocyte colony-stimulating factor; Peripheral blood stem cell transplantation; Upper extremity.

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Conflict of interest statement

CONFLICT OF INTEREST: No potential conflict of interest relevant to this article was reported.

Figures

Fig. 1
Fig. 1. A flowchart of this study. mPBMC, mobilized peripheral blood mononuclear cell; G-CSF, granulocyte-colony stimulating factor.
Fig. 2
Fig. 2. Changes of mean scores of subdomain of quality of upper extremity skills test in both groups during the study. M0, G-CSF injection time point; M1, mPBMC or placebo injection time point; M7, cross injection time point; M13, 6 months after cross injection; G-CSF, granulocyte-colony stimulating factor; mPBMC, mobilized peripheral blood mononuclear cells.
See this image and copyright information in PMC

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