Effect of effervescent buffered aspirin on prostaglandin synthesis by human gingival fibroblasts

Abstract

Cultured human gingival fibroblasts were incubated with 14C-arachidonic acid (AA) at 37 degrees C for 2 hours. The metabolites formed were extracted from the cell-free medium in methanol, separated and identified by thin layer chromatography, using two solvent systems that allowed resolution of cyclooxygenase and lipoxygenase products. The predominant cyclooxygenase products were PGE2 and 6-keto-PGF1 alpha. PGA2, PGF2 alpha, PGD2, 15-keto-PGE2, TXB2 were also detected in smaller amounts. No detectable radioactivity corresponding to lipoxygenase products 5-HETE, 12-HETE, and 15-HETE was found. Incubation of fibroblasts with effervescent buffered aspirin (EBA) (.02%, .04%, .06%), or sodium bicarbonate, citric acid and aspirin, individually, (in concentrations equivalent to those present in EBA) resulted in stimulation of synthesis of PGs except PGE2 which was inhibited by EBA and aspirin.