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. 1987 Dec;17(1-4):453-64.
doi: 10.1016/0165-2427(87)90161-9.

Rapid detection of viral-specific antibodies by enzyme-linked immunosorbent assay (ELISA)

S Winston  1 , S Fiscus, L Hesterberg, T Matsushita, M Mildbrand, J Porter, Y Teramoto
Affiliations

Rapid detection of viral-specific antibodies by enzyme-linked immunosorbent assay (ELISA)

S Winston et al. Vet Immunol Immunopathol. 1987 Dec.

Abstract

The development of three separate rapid ELISAs for detecting antibodies in host serum to three different viruses is described. These include: 1. A direct antigen assay using enzyme labelled anti-canine Ig for detecting antibodies to canine parvovirus, 2. A competitive ELISA using a feline infectious peritonitis virus-specific monoclonal antibody labelled with enzyme, and 3. A competitive ELISA using an equine infectious anemia virus-specific monoclonal antibody and enzyme labelled antigen, p. 26. The utility and benefits of each of the three approaches is emphasized.

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References

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