Polyoma integrates readily in mouse cellular DNA

Abstract

Although the natural host of polyoma virus is the mouse, its integration in cellular DNA has been investigated almost exclusively in rat cells. We report here studies on the integration of polyoma in mouse cells. We introduced the polyoma virus genome in two different mouse cell lines as an unselected genetic marker, by cotransfection with the tk gene of herpes simplex virus or the neo gene of E. coli. The number of TK+ or G418R clones obtained was reduced up to 50 fold by the presence of the polyoma genome. The gene coding for the early protein large T of polyoma was necessary and sufficient to produce this reduction. However, this effect appeared to be independent of polyoma replication. Surprisingly, all of the 33 clones analysed that had survived cotransfection with polyoma contained polyoma DNA integrated in their genome. Furthermore, in over 50% of these clones, the entire polyoma genome had been integrated. We conclude that polyoma integrates readily in mouse cellular DNA.