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. 1987 Dec;53(12):2967-71.
doi: 10.1128/aem.53.12.2967-2971.1987.

Production of cytopathology in FRhK-4 cells by BS-C-1-passaged hepatitis A virus

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Production of cytopathology in FRhK-4 cells by BS-C-1-passaged hepatitis A virus

A M Nasser et al. Appl Environ Microbiol. 1987 Dec.

Abstract

Cytopathic effects were produced in fetal rhesus monkey kidney (FRhK-4) cells 7 days postinfection by a serially BS-C-1-passaged strain of hepatitis A virus. Typical enterovirus cytopathology was produced by the HM-175 strain after 15 passages at 7-day intervals in BS-C-1 cells. No cytopathic effects were obtained after neutralization of virus with human anti-hepatitis A virus immunoglobulin G. Normal human serum had no effect on development of cytopathology. Maximum antigen and cDNA probe-based hybridization activity were associated with a CsCl gradient fraction having a density of 1.34 g/cm3. Large quantities of 27- to 30-nm virions typical of hepatitis A virus were associated with the same fraction. These data led to the conclusion that the observed cytopathology was caused by hepatitis A virus.

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