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. 2017:2017:9475074.
doi: 10.1155/2017/9475074. Epub 2017 Feb 16.

Antithrombotic Effect and Mechanism of Radix Paeoniae Rubra

Pingyao Xie  1 , Lili Cui  2 , Yuan Shan  2 , Wen-Yi Kang  1
Affiliations

Antithrombotic Effect and Mechanism of Radix Paeoniae Rubra

Pingyao Xie et al. Biomed Res Int. 2017.

Abstract

The compounds of Radix Paeoniae Rubra (RPR) were isolated and identified by bioassay-guided method, and antithrombotic effects and mechanism were investigated by the acute blood stasis rat model. The RPR extract was evaluated by APTT, TT, PT, and FIB assays in vitro. Results indicated that RPR extract exhibited the anticoagulant activity. In order to find active compounds, six compounds were isolated and identified, and four compounds, paeoniflorin (Pae), pentagalloylglucose (Pen), albiflorin (Ali), and protocatechuic acid (Pro), exhibited the anticoagulant activity in vitro. Therefore, the antithrombosis effects of RPR extract and four active compounds were investigated in vivo by measuring whole blood viscosity (WBV), plasma viscosity (PV), APTT, PT, TT, and FIB. Meanwhile, the levels of TXB2, 6-Keto-PGF1α , eNOS, and ET-1 were detected. Results suggested that RPR extract and four active compounds had the inhibition effect on thrombus formation, and the antithrombotic effects were associated with the regulation of vascular endothelium active substance, activating blood flow and anticoagulation effect.

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Conflict of interest statement

The authors declare that they have no conflict of interests.

Figures

Figure 1
Figure 1
Anticoagulation activity of RPR extract and six compounds in vitro (n = 4; ∗∗∗p < 0.001 or 0.001 < ∗∗p < 0.01 or ∗p < 0.05 versus control group; ###p < 0.001 or 0.001 < ##p < 0.01 or #p < 0.05 versus breviscapine).
Figure 2
Figure 2
Anticoagulation activity of RPR extract in vivo (n = 8; ∗∗∗p < 0.001 versus control group; ###p < 0.001 versus model group; △△△p < 0.001 versus aspirin; #p < 0.05 versus model group).
Figure 3
Figure 3
The effects of RPR treatment on eNOS and ET-1 level in thrombosis rats (n = 8; ∗∗∗p < 0.001 versus control group; ###p < 0.001 or 0.001 < ##p < 0.01 versus model group; △△△p < 0.001 or △p < 0.05 versus aspirin; #p < 0.05).
Figure 4
Figure 4
Anticoagulation activity of active compounds in vivo (n = 8; ∗∗∗p < 0.001 versus control group; ###p < 0.001 or #p < 0.05 versus model group; △△△p < 0.001 or △p < 0.05 versus Xdi; 0.001 < △△p < 0.01).
Figure 5
Figure 5
The effects of active compounds treatment on eNOS and ET-1 level in thrombosis rats (n = 8; ∗∗∗p < 0.001 versus control group; ###p < 0.001 or 0.001 < ##p < 0.01 versus model group; △△△p < 0.001 or △p < 0.05 versus Xdi; 0.001 < ∗∗p < 0.01 versus control group).
See this image and copyright information in PMC

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