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Review
. 2017:962:47-64.
doi: 10.1007/978-981-10-3233-2_4.

The Role of Runx1 in Embryonic Blood Cell Formation

Amanda D Yzaguirre  1 , Marella F T R de Bruijn  2 , Nancy A Speck  3
Affiliations
Review

The Role of Runx1 in Embryonic Blood Cell Formation

Amanda D Yzaguirre et al. Adv Exp Med Biol. 2017.

Abstract

The de novo generation of hematopoietic stem and progenitor cells (HSPC) occurs solely during embryogenesis from a population of epithelial cells called hemogenic endothelium (HE). During midgestation HE cells in multiple intra- and extraembryonic vascular beds leave the vessel wall as they transition into HSPCs in a process termed the endothelial to hematopoietic transition (EHT). Runx1 expression in HE cells orchestrates the transcriptional switch necessary for the transdifferentiation of endothelial cells into functional HSPCs. Runx1 is widely considered the master regulator of developmental hematopoiesis because it plays an essential function during specification of the hematopoietic lineage during embryogenesis. Here we review the role of Runx1 in embryonic HSPC formation, with a particular focus on its role in hemogenic endothelium.

Keywords: Hematopoiesis; Hematopoietic stem cell; Hemogenic endothelium; Runx1.

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Figures

Figure 1
Figure 1. Location of Runx1 expression and hemogenic endothelium in the mouse embryo
Confocal Z-projections of mouse embryos between embryonic day (E) 8.5 and E11.5 immunostained for the endothelial and hematopoietic marker CD31 (red) and Runx1 (green). Runx1 is expressed in hemogenic endothelium in the yolk sac (YS) at E8.5. At E9.5, Runx1 expression is prominent in the vitelline artery (VA) and umbilical artery (UA). An E10.5 embryo (head removed) shows Runx1 protein in the vitelline artery, umbilical artery, dorsal aorta (DA), and the site of colonization, the fetal liver (FL). al, allantois ; pDA, paired dorsal aorta. Scale bar = 500μm.
See this image and copyright information in PMC

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