Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1974 Dec;175(4):327-338.
doi: 10.1007/BF00574897.

Isolation of a fat body factor which stimulates evagination ofGalleria mellonella wing disksin vitro

J Benson  1   2 , H Oberlander  1   2 , Mosato Koreeda  1   2 , Koji Nakanishi  1   2
Affiliations

Isolation of a fat body factor which stimulates evagination ofGalleria mellonella wing disksin vitro

J Benson et al. Wilhelm Roux Arch Entwickl Mech Org. 1974 Dec.

Abstract

In previous papers we have reported that wing disks ofGalleria mellonella complete tracheole migration and evagination when they are cultured in a chemically defined medium containing alpha-ecdysone. The presence of larval fat body in the culture medium greatly improved the response of the disks. We have isolated this fat body factor from culture medium. It is dialyzable, heat stable (100° C, 5 min), and insensitive to pronase digestion. The fat body factor, recovered from thin layer plates, stimulated evagination in the absence of alpha-ecdysone. Furthermore, radiochromatography studies with tritiated alpha-ecdysone and spectral analysis of the factor indicated that it was neither a metabolite of alpha-ecdysone nor an ecdysone-like molecule.

PubMed Disclaimer

References

    1. Pure Appl Chem. 1971;25(1):167-95 - PubMed
    1. Naturwissenschaften. 1967 Sep;54(17):471-2 - PubMed
    1. Dev Biol. 1973 Aug;33(2):334-43 - PubMed
    1. J Insect Physiol. 1974 Apr;20(4):743-9 - PubMed
    1. J Insect Physiol. 1969 Feb;15(2):297-304 - PubMed