The effect of cytoskeletal disrupting agents on the morphological response of a clonedManduca sexta cell line to 20-hydroxy-ecdysone

Abstract

The embryonicManduca sexta cell line, MRRL-CH1 was cloned by dilution and single cell plating into conditioned medium, resulting in 6 cell clones with differing responses to the insect steroid hormone, 20-hydroxy-ecdysone. One of these clones, designated GV1, was studied with respect to cytoskeletal elements. Electron microscopic examination revealed that the microtubule networks became more extensive and organized increasingly parallel following exposure to the insect hormone 20-hydroxy-ecdysone, while inhibitors of microtubules were found to reverse the elongation response in the cells within 2.5 h. Agents affecting microfilaments (cytochalasins B and D) did not specifically alter the elongation response, but treatment with these agents did reveal increased cellular junctions as shown by increased cell aggregate size in hormone-treated cultures exposed to the cytochalasins.

Keywords: Clones; Cytoskeleton; Insect cell cultures; Manduca sexta.