doi: 10.1007/BF00868674.
RNA Polymerase B levels during the cell cycle ofPhysarum polycephalum
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- PMID: 28305171
- DOI: 10.1007/BF00868674
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RNA Polymerase B levels during the cell cycle ofPhysarum polycephalum
Wilehm Roux Arch Dev Biol.
1980 Oct.
Abstract
Tritiated α-amanitin has been used as a specific and sensitive probe to estimate the number of RNA polymerase B molecules in isolated nuclei, chromatin and nucleoids, obtained from macroplasmodia ofPhysarum polycephalum. During mitosis (metaphase±10 min) there is at least 10-fold less RNA polymerase B than at all phases of the cell cycle, even if DNA replication has been blocked in vivo. It is concluded that many of the RNA polymerase B molecules leave the chromatin during decondensation of the chromosomes in telophase of the synchronous nuclear division ofPhysarum.
Keywords: Cell cycle; Physarum; RNA polymerase B; [3H]α-amanitin binding.
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