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. 1980 Oct;189(3):165-169.
doi: 10.1007/BF00868674.

RNA Polymerase B levels during the cell cycle ofPhysarum polycephalum

Gérard Pierron  1 , Helmut W Sauer  1
Affiliations

RNA Polymerase B levels during the cell cycle ofPhysarum polycephalum

Gérard Pierron et al. Wilehm Roux Arch Dev Biol. 1980 Oct.

Abstract

Tritiated α-amanitin has been used as a specific and sensitive probe to estimate the number of RNA polymerase B molecules in isolated nuclei, chromatin and nucleoids, obtained from macroplasmodia ofPhysarum polycephalum. During mitosis (metaphase±10 min) there is at least 10-fold less RNA polymerase B than at all phases of the cell cycle, even if DNA replication has been blocked in vivo. It is concluded that many of the RNA polymerase B molecules leave the chromatin during decondensation of the chromosomes in telophase of the synchronous nuclear division ofPhysarum.

Keywords: Cell cycle; Physarum; RNA polymerase B; [3H]α-amanitin binding.

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References

    1. Nucleic Acids Res. 1978 Sep;5(9):3303-13 - PubMed
    1. J Cell Biol. 1979 Feb;80(2):451-64 - PubMed
    1. Eur J Biochem. 1980 Mar;104(2):423-31 - PubMed
    1. Wilehm Roux Arch Dev Biol. 1979 Sep;187(3):195-209 - PubMed
    1. Biochim Biophys Acta. 1964 Nov 15;91:399-405 - PubMed