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. 1986 Apr;195(3):182-185.
doi: 10.1007/BF02439436.

Difference in protein synthesis of ovaries indicates predetermined sex inChrysomya rufifacies (Diptera, Calliphoridae)

Christiane Kirchhoff  1
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Difference in protein synthesis of ovaries indicates predetermined sex inChrysomya rufifacies (Diptera, Calliphoridae)

Christiane Kirchhoff. Rouxs Arch Dev Biol. 1986 Apr.

Abstract

Sex in the monogenic blowflyChrysomya rufifacies is under the control of a germ-line autonomous maternal effect sex realizer. In order to identify the ovarian poly(A)+RNA that might be related to sex predetermination, we analysed the patterns of cell-free translation products of total poly(A)+RNA from female and male predetermined ovaries and oocytes. During vitellogenesis we observed one transient sex-linked difference in a stable pattern composed of more than 800 in vitro translated proteins. This difference, however, was no longer detectable in mature oocytes.

Keywords: Maternal effect; Oogenesis; Sex determination; Two-dimensional protein patterns.

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