Factors affecting DNA synthesis in an in vitro system

Abstract

Mononucleated striated muscle cells and one type of endoderm can be isolated from anthomedusae and cultivated in artificial sea-water. In the cultivated muscle the differentiated state is maintained. In the cultivated endoderm flagella are formed, but no new cell types differentiate and DNA synthesis or mitosis is not observed. When isolated muscle is grafted upon endoderm, regeneration or formation of new cell types is not observed. Following treatingment with bacterial collagenase DNA synthesis and flagellum formation are initiated in the isolated muscle; in the isolated endoderm, collagenase treatment has no effect. When striated muscle treated with collagenase is grafted upon endoderm, DNA synthesis is observed in the endoderm, and a regenerate is formed involving transdifferentiation. Although desmosomal contact between collagenase treated muslce and the endoderm is established, it is not sufficient to induce DNA synthesis; complete covering of the endoderm by the muscle is required.

Keywords: Anthomedusae; DNA systhesis; In vitro system; Transdifferentiation.