Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1988 Mar;197(2):124-127.
doi: 10.1007/BF00375935.

Flow cytometric determination of nuclear DNA content during differentiation (spherulation and germination) of the myxomycete Physarum polycephalum

I Lacorre-Arescaldino  1 , B Toublan  1 , C Pastisson  1
Affiliations

Flow cytometric determination of nuclear DNA content during differentiation (spherulation and germination) of the myxomycete Physarum polycephalum

I Lacorre-Arescaldino et al. Rouxs Arch Dev Biol. 1988 Mar.

Abstract

Using flow cytometry, spherulating nuclei of Physarum isolated at the beginning of spherule wall formation were found to exhibit a DNA content corresponding to the G2 phase of the cell cycle, although 8% lower. Before the first mitosis after spherule germination, a very slight incorporation of 3H thymidine into DNA was observed that was too weak to correspond to S phase, strongly suggesting that nuclei are stopped in G2 phase inside the spherules. The lower value of nuclear DNA content found using flow cytometry of germinating spherules may not be related to DNA quantity, but may be due to a difference in chromatin organization during growth or spherulation, resulting in interference with the staining.

Keywords: DNA-content; Differentiation; Flow cytometry; Physarum; Spherulation.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

References

    1. Prog Nucleic Acid Res Mol Biol. 1979;23:53-104 - PubMed
    1. Proc Natl Acad Sci U S A. 1965 May;53(5):924-31 - PubMed
    1. Exp Cell Res. 1983 Nov;149(1):57-67 - PubMed
    1. Exp Cell Res. 1980 Mar;126(1):137-42 - PubMed
    1. Biochim Biophys Acta. 1960 Feb 26;38:298-306 - PubMed