Mapping New Residents of the Mitochondrial Nucleoid
- PMID: 28306501
- DOI: 10.1016/j.chembiol.2017.03.006
Mapping New Residents of the Mitochondrial Nucleoid
Abstract
In this issue of Cell Chemical Biology, Han et al. (2017) used a powerful combination of quantitative ratiometric mass spectrometry with APEX peroxidase-catalyzed proximity biotinylation to selectively highlight proteins associated with mitochondrial DNA above the background of contaminants and matrix proteins. In addition to identifying novel nucleoid factors, this study extends the APEX strategy to the proteomic mapping of non-membrane-bound multiprotein complexes.
Copyright © 2017. Published by Elsevier Ltd.
Comment on
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Proximity Biotinylation as a Method for Mapping Proteins Associated with mtDNA in Living Cells.Cell Chem Biol. 2017 Mar 16;24(3):404-414. doi: 10.1016/j.chembiol.2017.02.002. Epub 2017 Feb 23. Cell Chem Biol. 2017. PMID: 28238724 Free PMC article.
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