Effect of low temperature on the photosynthetic metabolism of the C4 grass Echinochloa crus-galli

Abstract

CO₂ curves of photosynthesis and activities of the four C₄ enzymes and Ribulose bisphosphate carboxylase (RUBP_c) were compared in two populations of the C₄ grass Echinochloa crus-galli from contrasting thermal environments (Québec and Mississippi). Analyses were conducted both before and after 14 h of chilling at 7°C under high light conditions. This comparison provides the opportunity to assess which steps of the C₄ pathway are more susceptible to become limiting at low temperatures. Both populations maintained, after chilling, a pattern of CO₂ fixation typical of C₄ plants with photosynthesis saturating at low external CO₂ concentrations. However, the chilling treatment led to reductions in carbon uptake and in the activities of the C₄ enzymes. RUBP_c activity was not significantly affected by chilling. Reductions in photosynthesis and in C₄ enzyme activities following the chilling treatment were significantly larger for plants of the Mississippi population. The enzyme data suggest that two steps of the C₄ pathway, NADP⁺-malate dehydrogenase and pyruvate P_i dikinase, are likely to be associated with the reduction of CO₂ uptake in C₄ plants under cool conditions. When the experiment was replicated under enriched atmospheric CO₂ (675 μl l^-1 CO₂), similar differences were observed between the two populations. CO₂ enrichment resulted in an increase of activity for phospho-enol-pyruvate carboxylase and NADP⁺-malate dehydrogenase while activities of phospho-enol-pyruvate carboxylase and NADP⁺-malic enzyme were less reduced following chilling. Such an interaction was not observed for gas exchange parameters but net photosynthesis was lower when plants were grown under enriched CO₂.