Ecological tannin assays: a critique
- PMID: 28311906
- DOI: 10.1007/BF00377202
Ecological tannin assays: a critique
Abstract
The concept of protein precipitation potential has recently been introduced by Wisdom et al. (1987) as a means to combine chemical and protein precipitation assays of tannins for ecological studies. The definition of protein precipitation potential was not theoretically rigorous, and data analysis was obscure. Our attempts to repeat the tannin extraction procedure gave incomplete recovery (24% loss of quebracho) of condensed tannins, the only type considered by Wisdom et al. In contrast we found their method efficient for hydrolysable tannins (80% recovery of tannic acid) which were undetected by their chemical assay of tannins. Their protein precipitation assay was confounded by chemical interference from both types of tannins. We conclude with recommendations for this type of analysis.
Keywords: Methods; Protein assay; Tannin assay.
Similar articles
-
Ecological tannin assays : Evaluation of proanthocyanidins, protein binding assays and protein precipitating potential.Oecologia. 1987 Jun;72(3):395-401. doi: 10.1007/BF00377570. Oecologia. 1987. PMID: 28311136
-
A critical analysis of techniques for measuring tannins in ecological studies : II. Techniques for biochemically defining tannins.Oecologia. 1987 Apr;72(1):148-156. doi: 10.1007/BF00385059. Oecologia. 1987. PMID: 28312911
-
A critical analysis of techniques for measuring tannins in ecological studies : I. Techniques for chemically defining tannins.Oecologia. 1987 Apr;72(1):137-147. doi: 10.1007/BF00385058. Oecologia. 1987. PMID: 28312910
-
A modified method for determining tannin-protein precipitation capacity using accelerated solvent extraction (ASE) and microplate gel filtration.J Chem Ecol. 2006 Jun;32(6):1367-77. doi: 10.1007/s10886-006-9089-9. Epub 2006 May 23. J Chem Ecol. 2006. PMID: 16770724
-
Tannins in plant-herbivore interactions.Phytochemistry. 2011 Sep;72(13):1551-65. doi: 10.1016/j.phytochem.2011.01.040. Epub 2011 Feb 26. Phytochemistry. 2011. PMID: 21354580 Review.
Cited by
-
Foliar phenolics of nebraska sandhills prairie graminoids: Between-years, seasonal, and interspecific variation.J Chem Ecol. 1993 Sep;19(9):1861-74. doi: 10.1007/BF00983792. J Chem Ecol. 1993. PMID: 24249364
-
Linking chemical reactivity and protein precipitation to structural characteristics of foliar tannins.J Chem Ecol. 2003 Mar;29(3):703-30. doi: 10.1023/a:1022876804925. J Chem Ecol. 2003. PMID: 12757329
-
Preparation of an acid butanol standard from fresh apples.J Chem Ecol. 2010 May;36(5):453-60. doi: 10.1007/s10886-010-9784-4. Epub 2010 Apr 9. J Chem Ecol. 2010. PMID: 20379766
-
Methods and pitfalls of extracting condensed tannins and other phenolics from plants: Insights from investigations onEucalyptus leaves.J Chem Ecol. 1991 Jan;17(1):123-34. doi: 10.1007/BF00994426. J Chem Ecol. 1991. PMID: 24258438
-
Acacia senegal Extract Rejuvenates the Activity of Phenicols on Selected Enterobacteriaceae Multi Drug Resistant Strains.Antibiotics (Basel). 2020 Jun 13;9(6):323. doi: 10.3390/antibiotics9060323. Antibiotics (Basel). 2020. PMID: 32545716 Free PMC article.