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. 2017 Mar;41(1):243-246.
doi: 10.1007/s12639-016-0785-2. Epub 2016 Jun 22.

Comparison between conventional and molecular methods for diagnosis of bovine babesiosis (Babesia bovis infection) in tick infested cattle in upper Egypt

Amira A T Al-Hosary  1
Affiliations

Comparison between conventional and molecular methods for diagnosis of bovine babesiosis (Babesia bovis infection) in tick infested cattle in upper Egypt

Amira A T Al-Hosary. J Parasit Dis. 2017 Mar.

Abstract

Ticks and tick-borne diseases are the main problems affecting the livestock production in Egypt. Bovine babesiosis has adverse effects on the animal health and production. A comparison of Giemsa stained blood smears, polymerase chain reaction (PCR) and nested PCR (nPCR) assays for detection of Babesia bovis infection in Egyptian Baladi cattle (Bos taurus) in reference to reverse line blot was carried out. The sensitivity of PCR and nested PCR (nPCR) assays were 65 and 100 % respectively. Giemsa stained blood smears showed the lowest sensitivity (30 %). According to these results using of PCR and nPCR target for B. bovis, [BBOV-IV005650 (BV5650)] gene are suitable for diagnosis of B. bovis infection. The 18Ss rRNA partial sequence confirmed that all the positive samples were Babesia bovis and all of them were deposited in the GenBank databases (Accession No: KM455548, KM455549 and KM455550).

Keywords: 18Ss rRNA; Babesia bovis; Giemsa; PCR; nPCR.

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Conflict of interest statement

Author confirmed that there is no conflict of interest.

Figures

Fig. 1
Fig. 1
Giemsa stained blood smear showing Babesia bovis piroplasms
Fig. 2
Fig. 2
Babesia bovis PCR products. Lane M: DNA ladder 100 bp, Lanes 1, 3, 5, 7 and 8: positive PCR with bands of 720 bp
Fig. 3
Fig. 3
Babesia bovis nested PCR products. Lane M: DNA ladder 100 bp, Lanes 1, 3, 5, 7, 8 and 10: positive PCR with bands of 561 bp
Fig. 4
Fig. 4
Babesia bovis RLB-PCR products. Lane M: DNA ladder 100 bp, Lanes 1, 4, 6, 7 and 10: positive PCR
Fig. 5
Fig. 5
Babesia bovis nested RLB-PCR products. Lane M: DNA ladder 100 bp, Lanes 1, 2, 4, 6, 7 and 10: positive PCRs both of them gave bands of 460 and 520 bp
See this image and copyright information in PMC

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