Identification and genetic characterization of porcine circovirus type 3 in China

Abstract

A novel circovirus called porcine circovirus type 3 (PCV3) was recently reported to exist in the USA. This circovirus is associated with porcine dermatitis, nephropathy syndrome and reproductive failure. This study reports on the first identification, widely epidemic, different phylogenetic clusters, potential role in sow reproductive failure and possible origins of PCV3 in China.

Keywords: China; complete genome; genetic characteristic; identification; porcine circovirus type 3.

Figure 1

(a) Phylogenetic tree of the complete genome of different circovirus species. (b) Phylogenetic tree of partial capsid gene of different Chinese PCV3 isolates with a USA PCV3 isolates PCV3‐USA‐MO2015 (KX778720.1). The phylogenetic tree was constructed using the p‐distance‐based neighbour‐joining method in MEGA 6.06 software. Bootstrap values were calculated with 1,000 replicates. Black solid circles indicate the strains from China. Black solid square indicates the strain from USA. Black solid triangles indicate the bat circovirus strains from China. Scale bars indicate nucleotide substitutions per site.

Figure 2

Similarity plot of the whole genome of 10 PCV3 isolates with 5 Bat circovirus isolates. The Chinese PCV3 isolates, PCV3/CN/Fujian‐5/2016, was set as query strain. The similarity plot was constructed using the two‐parameter (Kimura) distance model with a sliding window of 200 bp and step size of 20 bp. The vertical and horizontal axes indicate the nucleotide similarity per cent and nucleotide position (bp) in the graph, respectively.