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. 2017 Apr 6;66(1):38-49.e6.
doi: 10.1016/j.molcel.2017.02.009. Epub 2017 Mar 16.

Genome-wide Analysis of RNA Polymerase II Termination at Protein-Coding Genes

Carlo Baejen  1 , Jessica Andreani  1 , Phillipp Torkler  1 , Sofia Battaglia  1 , Bjoern Schwalb  1 , Michael Lidschreiber  2 , Kerstin C Maier  1 , Andrea Boltendahl  1 , Petra Rus  1 , Stephanie Esslinger  3 , Johannes Söding  4 , Patrick Cramer  5
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Free article

Genome-wide Analysis of RNA Polymerase II Termination at Protein-Coding Genes

Carlo Baejen et al. Mol Cell. .
Free article

Abstract

At the end of protein-coding genes, RNA polymerase (Pol) II undergoes a concerted transition that involves 3'-processing of the pre-mRNA and transcription termination. Here, we present a genome-wide analysis of the 3'-transition in budding yeast. We find that the 3'-transition globally requires the Pol II elongation factor Spt5 and factors involved in the recognition of the polyadenylation (pA) site and in endonucleolytic RNA cleavage. Pol II release from DNA occurs in a narrow termination window downstream of the pA site and requires the "torpedo" exonuclease Rat1 (XRN2 in human). The Rat1-interacting factor Rai1 contributes to RNA degradation downstream of the pA site. Defects in the 3'-transition can result in increased transcription at downstream genes.

Keywords: RNA polymerase II; Torpedo nuclease; poly-adenylation; pre-mRNA 3′-processing; transcription termination.

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