Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Comparative Study
. 2017 Mar 21;18(1):115.
doi: 10.1186/s12891-017-1466-8.

Single nucleotide polymorphisms in the CD40 gene associate with the disease susceptibility and severity in knee osteoarthritis in the Chinese Han population: a case-control study

Zhen-Han Deng  1 , Ming-Hua Sun  2 , Yu-Sheng Li  1 , Wei Luo  1 , Fang-Jie Zhang  3 , Jian Tian  1 , Ping Wu  4 , Wen-Feng Xiao  5
Affiliations
Comparative Study

Single nucleotide polymorphisms in the CD40 gene associate with the disease susceptibility and severity in knee osteoarthritis in the Chinese Han population: a case-control study

Zhen-Han Deng et al. BMC Musculoskelet Disord. .

Abstract

Background: This study explored the association between single nucleotide polymorphisms (SNPs) in the CD40 gene, rs4810485 G > T and rs1883832 C > T, as well as disease susceptibility and severity in knee osteoarthritis (KOA) in the Chinese Han population.

Method: Peripheral venous blood was collected from 133 KOA patients (KOA group) and 143 healthy people (control group) from December 2012 to November 2013. The patients in the KOA group were classified into mild, moderate and severe groups according to disease severity. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was used to test the genotypes of all subjects. Binary logistic regression analyses were performed to analyze the risk factors for KOA.

Results: The KOA group was significantly different from the control group in living environment (P < 0.05). The KOA group had a lower frequency of TT genotype and T allele distribution of rs4810485 G > T compared with the control group, and rs4810485 G > T TT genotype and T allele may associate with low incidence of KOA (all P < 0.05). Besides, T allele and mutant homozygous TT genotype of rs1883832 C > T increased the susceptibility to KOA. Genotype and allele distribution of rs4810485 G > T and rs1883832 C > T were significantly different among the mild, moderate and severe groups (P < 0.05). There were more patients with rs4810485 G > T GG genotype and rs1883832 C > T TT genotype in the severe group than other genotypes of these two SNPs. According to binary logistic regression analysis, rs4810485 G > T TT genotype could alleviate disease severity in KOA, rs1883832 C > T TT genotype increase the severity of KOA and living environment is an important external factor that affects KOA severity.

Conclusions: These data provide evidences that rs4810485 G > T and rs1883832 C > T in the CD40 gene may be associated with disease susceptibility and severity in KOA.

Keywords: CD40; Knee osteoarthritis; Rs1883832 C > T; Rs4810485 G > T; Severity; Single nucleotide polymorphism; Susceptibility.

PubMed Disclaimer

Figures

Fig. 1
Fig. 1
Electrophoresis map of CD40 rs4810485 G > T and rs1883832 C > T. Note: a, CD40 rs4810485 G > T polymorphism: 1 and 5, GG genotype; 2 and 4, TT genotype; 3 and 6, GT genotype; b, CD40 rs1883832 C > T polymorphism: 1 and 5, TT genotype; 2 and 6, CT genotype; 3 and 4, CC genotype; CD40, Cluster of Differentiation 40
See this image and copyright information in PMC

References

    1. Kirkley A, Birmingham TB, Litchfield RB, Giffin JR, Willits KR, Wong CJ, Feagan BG, Donner A, Griffin SH, D’Ascanio LM, Pope JE, Fowler PJ. A randomized trial of arthroscopic surgery for osteoarthritis of the knee. N Engl J Med. 2008;359(11):1097–1107. doi: 10.1056/NEJMoa0708333. - DOI - PubMed
    1. Blagojevic M, Jinks C, Jeffery A, Jordan KP. Risk factors for onset of osteoarthritis of the knee in older adults: a systematic review and meta-analysis. Osteoarthritis Cartilage. 2010;18(1):24–33. doi: 10.1016/j.joca.2009.08.010. - DOI - PubMed
    1. Zhang W, Nuki G, Moskowitz RW, Abramson S, Altman RD, Arden NK, Bierma-Zeinstra S, Brandt KD, Croft P, Doherty M, Dougados M, Hochberg M, Hunter DJ, Kwoh K, Lohmander LS, Tugwell P. OARSI recommendations for the management of hip and knee osteoarthritis: part III: Changes in evidence following systematic cumulative update of research published through January 2009. Osteoarthritis Cartilage. 2010;18(4):476–499. doi: 10.1016/j.joca.2010.01.013. - DOI - PubMed
    1. Williams A, Qian Y, Golla S, Chu CR. UTE-T2 * mapping detects sub-clinical meniscus injury after anterior cruciate ligament tear. Osteoarthritis Cartilage. 2012;20(6):486–494. doi: 10.1016/j.joca.2012.01.009. - DOI - PMC - PubMed
    1. Oiestad BE, Engebretsen L, Storheim K, Risberg MA. Knee osteoarthritis after anterior cruciate ligament injury: a systematic review. Am J Sports Med. 2009;37(7):1434–1443. doi: 10.1177/0363546509338827. - DOI - PubMed

Publication types

MeSH terms

LinkOut - more resources