Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1987 Dec 20;6(13):4143-8.
doi: 10.1002/j.1460-2075.1987.tb02760.x.

Homologous recombination in the Dictyostelium alpha-actinin gene leads to an altered mRNA and lack of the protein

W Witke  1 , W Nellen, A Noegel
Affiliations

Homologous recombination in the Dictyostelium alpha-actinin gene leads to an altered mRNA and lack of the protein

W Witke et al. EMBO J. .

Abstract

Mutation of the alpha-actinin gene in Dictyostelium has been achieved by transforming cells with the Dictyostelium transformation vector pDNeoII containing a 1.2 kb fragment of the alpha-actinin gene. Transformants deficient in alpha-actinin, an actin-binding protein, produced an altered mRNA that lacked the 3' portion of the coding region. The defect in alpha-actinin production was not due to integration of the vector within the gene, but was apparently caused by errors produced during homologous recombination between the introduced alpha-actinin sequence and its complementary sequence in the coding region of the endogenous gene.

PubMed Disclaimer

References

    1. Mol Cell Biol. 1984 Dec;4(12):2890-8 - PubMed
    1. Nature. 1970 Aug 15;227(5259):680-5 - PubMed
    1. J Cell Biochem. 1984;24(1):27-53 - PubMed
    1. Gene. 1984 Dec;32(3):481-5 - PubMed
    1. FEBS Lett. 1987 Sep 14;221(2):391-6 - PubMed

Publication types