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. 1987;60(2-3):183-9.
doi: 10.1016/0378-1119(87)90226-5.

Purification of hepatitis B virus gene X product synthesized in Escherichia coli and its detection in a human hepatoblastoma cell line producing hepatitis B virus

O Chisaka  1 , K Araki, T Ochiya, T Tsurimoto, W Hiranyawasitte-Attatippaholkun, N Yanaihara, K Matsubara
Affiliations

Purification of hepatitis B virus gene X product synthesized in Escherichia coli and its detection in a human hepatoblastoma cell line producing hepatitis B virus

O Chisaka et al. Gene. 1987.

Abstract

A fused gene containing 94% of the hepatitis B virus (HBV) open reading frame X was expressed in Escherichia coli, and its 17-kDa product was purified by ion-exchange chromatography. Antibody elicited against the X-gene product reacted with materials proximal to the nuclear membrane of a human hepatoblastoma cell line producing HBV particles. No such reaction was observed with the same cell line that did not produce HBV particles.

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