Graphene Oxide Quantum Dots as Novel Nanozymes for Alcohol Intoxication

Abstract

Alcohol overconsumption as a worldwide issue results in alcoholic liver disease (ALD), such as steatosis, alcoholic hepatitis, and cirrhosis. The treatment of ALD has been widely investigated but remains challenging. In this work, the protective effects of graphene oxide quantum dots (GOQDs) as novel nanozymes against alcohol overconsumption are discovered, and the specific mechanisms underlying these effects are elucidated via omics analysis. GOQDs dramatically alleviate the reduction of cell viability induced by ethanol and can act as nanozymes to accelerate ethanol metabolism and avoid the accumulation of toxic intermediates in cells. Mitochondrial damage and the excessive generation of free radicals were mitigated by GOQDs. The mechanisms underlying the cellular protective effects were also related to alterations in metabolic and protein signals, especially those involved in lipid metabolism. The moderately increased autophagy induced by GOQDs explained the removal of accumulated lipids and the subsequent elimination of excessive GOQDs. These findings suggest that GOQDs have an antagonistic capacity against the adverse effects caused by ethanol and provide new insights into the direct applications of GOQDs. In addition to traditional antioxidation, this work also establishes metabolomics and proteomics techniques as effective tools to discover the multiple functions of nanozymes.

Keywords: artificial enzyme; ethanol; graphene; hepatocyte; nanozyme; oxidative stress.