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. 2014 Oct;4(5):507-512.
doi: 10.1007/s13205-013-0182-9. Epub 2013 Nov 13.

Biodegradation of poly (ε-caprolactone) (PCL) film and foam plastic by Pseudozyma japonica sp. nov., a novel cutinolytic ustilaginomycetous yeast species

Fatma F Abdel-Motaal  1 , Magdi A El-Sayed  2 , Soad A El-Zayat  1 , Shin-Ichi Ito  3
Affiliations

Biodegradation of poly (ε-caprolactone) (PCL) film and foam plastic by Pseudozyma japonica sp. nov., a novel cutinolytic ustilaginomycetous yeast species

Fatma F Abdel-Motaal et al. 3 Biotech. 2014 Oct.

Abstract

Aliphatic polyesters poly (ε-caprolactone) (PCL) and foam plastic have been shown to be biodegradable by microorganisms, which possess cutinolytic enzymes. Pseudozyma japonica-Y7-09, showed both high growth and enzyme activity on Yeast malt (YM) medium fed with PCL film than on YM medium. The hydrolytic enzyme activity of the culture on p-nitrophenyl butyrate indicated the occurrence of cutinase enzyme. This activity was confirmed by the degradation of PCL film which reached to the maximum (93.33 %) at 15 days and the degradation of foam plastic which reached 43.2 % at 30 days. These results suggest that the extracellular cutinase enzyme of Pseudozyma japonica-Y7-09 may be useful for the biological degradation of plastic wastes.

Keywords: Foam degradation; PCL degradation; Plastic degradation; Pseudozyma; Yeast.

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Figures

Fig. 1
Fig. 1
Growth measured by culture turbidity (OD600nm) in control (culture in YM) and culture in YM fed with PCL film (YM and PCL film)
Fig. 2
Fig. 2
Activity of enzyme secreted by Pseudozyma japonica-Y7-09 in yeast malt culture fed and that not fed with PCL film
Fig. 3
Fig. 3
Total protein concentration secreted by Pseudozyma japonica-Y7-09 in yeast malt culture fed and that not fed with PCL film
Fig. 4
Fig. 4
a Degradation of PCL film after 0, 3, 5, 8, 12 and 15 days incubation time by Pseudozyma japonica-Y7-09 comparing with control. b Weight loss percentage of PCL film after 0, 3, 5, 8, 12 and 15 days incubation time by Pseudozyma japonica-Y7-09 comparing with control
Fig. 5
Fig. 5
Degradation of foam plastic by Pseudozyma japonica-Y7-09 incubated in yeast malt media for 30 days
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