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. 2015 Dec;5(6):933-938.
doi: 10.1007/s13205-015-0295-4. Epub 2015 Mar 17.

Validation of dbEST-SSRs and transferability of some other solanaceous species SSR in ashwagandha [Withania Somnifera (L.) Dunal]

Eva K Parmar  1 , Ranbir S Fougat  1 , Chandni B Patel  1 , Harshvardhan N Zala  1 , Mahesh A Patel  1 , Swati K Patel  2 , Sushil Kumar  3
Affiliations

Validation of dbEST-SSRs and transferability of some other solanaceous species SSR in ashwagandha [Withania Somnifera (L.) Dunal]

Eva K Parmar et al. 3 Biotech. 2015 Dec.

Abstract

Cross-species transferability and expressed sequence tags (ESTs) in public databases are cost-effective means for developing simple sequence repeats (SSRs) for less-studied species like medicinal plants. In this study, 11 EST-SSR markers developed from 742 available ESTs of Withania Somnifera EST sequences and 95 SSR primer pairs derived from other solanaceous crops (tomato, eggplant, chili, and tobacco) were utilized for their amplification and validation. Out of 11, 10 EST-SSRs showed good amplification quality and produced 13 loci with a product size ranging between 167 and 291 bp. Similarly, of the 95 cross-genera SSR loci assayed, 20 (21 %) markers showed the transferability of 5, 27, 32, and 14.2 % from eggplant, chili, tomato, and tobacco, respectively, to ashwagandha. In toto, these 30 SSR markers reported here will be valuable resources and may be applicable for the analysis of intra- and inter-specific genetic diversity in ashwagandha for which till date no information about SSR is available.

Keywords: Ashwagandha; Cross-genera transferability; Expressed sequence tags; Genomic SSR; Medicinal plant; Withania somnifera.

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Figures

Fig. 1
Fig. 1
Amplification profile of (a) CaES3962 SSR through cross-species amplification and (b) WS_SSR08, a dbEST-SSR, in 12 genotypes of Withania somnifera; lane 1 100-bp ladder DNA marker; lane 2–13 AWR 1, AWR 2, AWR 3, AWR 4, AWR 5, AWR 6, AWS 3-49, AWS 21, AWS 54, AWS 60/1, AWS 62, and AWS 19, respectively
See this image and copyright information in PMC

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