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Comparative Study
. 1988 Apr 6;108(1-2):33-7.
doi: 10.1016/0022-1759(88)90399-7.

Rapid, sensitive detection of plasma IL-1 by extraction and bioassay

J J Series  1 , D Shapiro, I Cameron, J Smith, W D Fraser, O J Garden, A Shenkin
Affiliations
Comparative Study

Rapid, sensitive detection of plasma IL-1 by extraction and bioassay

J J Series et al. J Immunol Methods. .

Abstract

A method for separating IL-1 from plasma inhibitors by silica extraction has been developed and coupled to a highly sensitive bioassay using the LBRM TG6 cell line and the I1-2 dependent HT2A cell line. Using this assay we have detected IL-1 activity in plasma from patients undergoing elective surgery.

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