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. 2016 Jun;6(1):45.
doi: 10.1007/s13205-016-0372-3. Epub 2016 Feb 4.

Novel pathway of cypermethrin biodegradation in a Bacillus sp. strain SG2 isolated from cypermethrin-contaminated agriculture field

Pankaj  1 , Anita Sharma  2 , Saurabh Gangola  2 , Priyanka Khati  2 , Govind Kumar  2 , Anjana Srivastava  3
Affiliations

Novel pathway of cypermethrin biodegradation in a Bacillus sp. strain SG2 isolated from cypermethrin-contaminated agriculture field

Pankaj et al. 3 Biotech. 2016 Jun.

Abstract

Pesticides belonging to pyrethroid group are widely used in agricultural fields to check pest infestation in different crops for enhanced food production. In spite of beneficial effects, non-judicious use of pesticides imposes harmful effect on human health as their residues reach different food materials and ground water via leaching, percolation and bioaccumulation. Looking into the potential of microbial degradation of toxic compounds under natural environment, a cypermethrin-degrading Bacillus sp. was isolated from pesticide-contaminated soil of a rice field of Distt. Udham Singh Nagar, Uttarakhand, India. The bacteria degraded the compound up to 81.6 % within 15 days under standard growth conditions (temperature 32 °C pH 7 and shaking at 116 rpm) in minimal medium. Analysis of intermediate compounds of biodegraded cypermethrin revealed that the bacteria opted a new pathway for cypermethrin degradation. GC-MS analysis of biodegraded cypermethrin showed the presence of 4-propylbenzoate, 4-propylbenzaldehyde, phenol M-tert-butyl and 1-dodecanol, etc. which was not reported earlier in cypermethrin metabolism; hence a novel biodegradation pathway of cypermethrin with Bacillus sp. strain SG2 is proposed in this study.

Keywords: Bacillus sp.; Biodegradation; Cypermethrin; GC–MS; Pyrethroid.

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Conflict of interest statement

Author declares no conflict of interest.

Figures

Fig. 1
Fig. 1
Phylogenetic analysis of SG2 strain and related strains on the basis of 16S rDNA using MEGA 5.0 software. The numbers in parentheses represent the sequence accession number in Genbank. Bar represents sequence divergence
Fig. 2
Fig. 2
Utilization of cypermethrin by strain SG2 (filled circle) growth of SG2, (filled square) utilization kinetics of cypermethrin, (filled triangle) Control
Fig. 3
Fig. 3
Response surface plot showing effect of temperature, pH and shaking speed on cypermethrin biodegradation (where A temperature, B pH, C shaking speed)
Fig. 4
Fig. 4
FT-IR spectra of cypermethrin. a Cypermethrin control. b Cpermethrin treated with SG2
Fig. 5
Fig. 5
GC-MS spectra of the cypermethrin with strain SG2
Fig. 6
Fig. 6
Proposed pathway of Cypermethrin degradation with SG2 strain
See this image and copyright information in PMC

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