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. 2016 Dec;6(2):165.
doi: 10.1007/s13205-016-0480-0. Epub 2016 Aug 11.

Biocatalytic activity of Aspergillus niger xylanase in paper pulp biobleaching

A Sridevi  1 , A Sandhya  1 , G Ramanjaneyulu  2 , G Narasimha  3 , P Suvarnalatha Devi  4
Affiliations

Biocatalytic activity of Aspergillus niger xylanase in paper pulp biobleaching

A Sridevi et al. 3 Biotech. 2016 Dec.

Abstract

Xylanase is a hemicellulase enzyme that catalyses the hydrolysis of xylan to xylose which is widely used in processing of feed, pulp and paper. It is produced by many microorganisms especially filamentous fungi like Trichoderma and Aspergillus. A potential xylanolytic fungal isolate Aspergillus niger was isolated from forest soils of Tirumala, AP, India, and its crude enzyme was checked for its potential in paper bleaching. Under submerged fermentation, production of xylanase, cellulase, biomass, total protein and sugar released were analysed after 7 days of incubation at room temperature. Maximum enzyme activity was recorded on the fifth day of incubation, biomass after the seventh day, total protein and sugar released on the sixth day of incubation. Enzyme pretreatment of paper reduced 3.5 points in kappa number, 3.1 points increase in brightness and removal of chromophores and hydrophobic compounds. The FTIR and SEM analysis of enzyme-treated sample had shown modification in surface morphology and functional groups. These results clearly demonstrated that the xylanase produced by A. niger was effective as a pulp biobleaching agent which can be used on an industrial scale.

Keywords: Biobleaching; Effluent characteristics; FTIR; SEM; Xylanase.

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Conflict of interest statement

Authors have no conflict of interest.

Figures

Fig. 1
Fig. 1
Isolation and screening of A. niger for production of xylanase
Fig. 2
Fig. 2
Phylogenetic tree of potential isolate constructed using NCBI
Fig. 3
Fig. 3
a Untreated paper pulp FTIR spectra. b Enzyme-treated paper pulp
Fig. 4
Fig. 4
a Untreated paper pulp SEM image. b Enzyme-treated paper pulp
See this image and copyright information in PMC

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