Biomass-derived molecules modulate the behavior of Streptomyces coelicolor for antibiotic production

Affiliations

¹ Department of Microbial Engineering, College of Engineering, Konkuk University, Seoul, 143-701, South Korea.
² Institute for Ubiquitous Information Technology and Applications Konkuk University, Seoul, 143-701, South Korea.
³ IT Convergence Materials R&BD Group, Chungcheong Regional Division, Korea Institute of Industrial Technology (KITECH), 35-3 Hongchon-ri, Ipjang-myun, Seobuk-gu, Chonan-si, Chungnam, 330-825, South Korea.
⁴ Biotechnology Process Engineering Center, Korea Research Institute Bioscience Biotechnology (KRIBB), Gwahangno, Yuseong-Gu, Daejeon, 305-806, South Korea.
⁵ Department of Biological and Chemical Engineering, Hongik University, Sejong Ro 2639, Jochiwon, Sejong, South Korea.
⁶ Department of Microbial Engineering, College of Engineering, Konkuk University, Seoul, 143-701, South Korea. seokor@konkuk.ac.kr.
⁷ Institute for Ubiquitous Information Technology and Applications Konkuk University, Seoul, 143-701, South Korea. seokor@konkuk.ac.kr.

PMID: 28330295
PMCID: PMC5065882
DOI: 10.1007/s13205-016-0539-y

Biomass-derived molecules modulate the behavior of Streptomyces coelicolor for antibiotic production

Shashi Kant Bhatia et al. 3 Biotech. 2016 Dec.

. 2016 Dec;6(2):223.

doi: 10.1007/s13205-016-0539-y. Epub 2016 Oct 15.

Authors

Affiliations

¹ Department of Microbial Engineering, College of Engineering, Konkuk University, Seoul, 143-701, South Korea.
² Institute for Ubiquitous Information Technology and Applications Konkuk University, Seoul, 143-701, South Korea.
³ IT Convergence Materials R&BD Group, Chungcheong Regional Division, Korea Institute of Industrial Technology (KITECH), 35-3 Hongchon-ri, Ipjang-myun, Seobuk-gu, Chonan-si, Chungnam, 330-825, South Korea.
⁴ Biotechnology Process Engineering Center, Korea Research Institute Bioscience Biotechnology (KRIBB), Gwahangno, Yuseong-Gu, Daejeon, 305-806, South Korea.
⁵ Department of Biological and Chemical Engineering, Hongik University, Sejong Ro 2639, Jochiwon, Sejong, South Korea.
⁶ Department of Microbial Engineering, College of Engineering, Konkuk University, Seoul, 143-701, South Korea. seokor@konkuk.ac.kr.
⁷ Institute for Ubiquitous Information Technology and Applications Konkuk University, Seoul, 143-701, South Korea. seokor@konkuk.ac.kr.

PMID: 28330295
PMCID: PMC5065882
DOI: 10.1007/s13205-016-0539-y

Abstract

Various chemicals, i.e., furfural, vanillin, 4-hydroxybenzaldehyde and acetate produced during the pretreatment of biomass affect microbial fermentation. In this study, effect of vanillin, 4-hydroxybenzaldehyde and acetate on antibiotic production in Streptomyces coelicolor is investigated. IC ₅₀ value of vanillin, 4-hydroxybenzaldehyde and acetate was recorded as 5, 11.3 and 115 mM, respectively. Vanillin was found as a very effective molecule, and it completely abolished antibiotic (undecylprodigiosin and actinorhodin) production at 1 mM concentration, while 4-hydroxybenzaldehyde and acetate have little effect. Microscopic analysis with field emission scanning electron microscopy (FESEM) showed that addition of vanillin inhibits mycelia formation and increases differentiation of S. coelicolor cells. Vanillin increases expression of genes responsible for sporulation (ssgA) and decreases expression of antibiotic transcriptional regulator (redD and actII-orf4), while it has no effect on genes related to the mycelia formation (bldA and bldN) and quorum sensing (scbA and scbR). Vanillin does not affect the glycolysis process, but may affect acetate and pyruvate accumulation which leads to increase in fatty acid accumulation. The production of antibiotics using biomass hydrolysates can be quite complex due to the presence of exogenous chemicals such as furfural and vanillin, and needs further detailed study.

Keywords: Antibiotic; Biomass; Furfural; Streptomyces coelicolor; Vanillin.

PubMed Disclaimer

Conflict of interest statement

The authors declare that they have no conflict of interest.

Figures

**Fig. 1**
Effect of biomass-derived chemicals a vanillin, b 4-hydroxybenzaldehyde (4-HB) and c acetate on *S. coelicolor* growth and antibiotic production. *S. coelicolor* was cultured in M9 media with 1 % glucose and various concentrations of inhibitors

**Fig. 2**
Field emission scanning electron microscopy (FESEM) of *S. coelicolor*. a Control: normal cell morphology with mycelia; b with vanillin: cells are *round shaped* and mycelia formation is inhibited

See this image and copyright information in PMC

Cited by

Insights into Streptomyces coelicolor A3(2) growth and pigment formation with high-throughput online monitoring.
Finger M, Sentek F, Hartmann L, Palacio-Barrera AM, Schlembach I, Rosenbaum MA, Büchs J. Finger M, et al. Eng Life Sci. 2022 Apr 28;23(1):e2100151. doi: 10.1002/elsc.202100151. eCollection 2023 Jan. Eng Life Sci. 2022. PMID: 36619878 Free PMC article.
SCO6992, a Protein with β-Glucuronidase Activity, Complements a Mutation at the absR Locus and Promotes Antibiotic Biosynthesis in Streptomyces coelicolor.
Jin XM, Choi MY, Tsevelkhoroloo M, Park U, Suh JW, Hong SK. Jin XM, et al. J Microbiol Biotechnol. 2021 Nov 28;31(11):1591-1600. doi: 10.4014/jmb.2108.08001. J Microbiol Biotechnol. 2021. PMID: 34584035 Free PMC article.
Metabolic engineering of Streptomyces to enhance the synthesis of valuable natural products.
Xu Z, Ji L, Tang W, Guo L, Gao C, Chen X, Liu J, Hu G, Liu L. Xu Z, et al. Eng Microbiol. 2022 Apr 23;2(2):100022. doi: 10.1016/j.engmic.2022.100022. eCollection 2022 Jun. Eng Microbiol. 2022. PMID: 39628845 Free PMC article. Review.
Bioengineering of anaerobic digestion for volatile fatty acids, hydrogen or methane production: A critical review.
Wainaina S, Lukitawesa, Kumar Awasthi M, Taherzadeh MJ. Wainaina S, et al. Bioengineered. 2019 Dec;10(1):437-458. doi: 10.1080/21655979.2019.1673937. Bioengineered. 2019. PMID: 31570035 Free PMC article. Review.

References

1. Akillioglu HG, Mogol BA, Gokmen V. Degradation of 5-hydroxymethylfurfural during yeast fermentation. Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 2011;28:1629–1635. - PubMed
1. Allen SA, et al. Furfural induces reactive oxygen species accumulation and cellular damage in Saccharomyces cerevisiae. Biotechnol Biofuels. 2010;3:1–10. doi: 10.1186/1754-6834-3-2. - DOI - PMC - PubMed
1. Bhatia SK, Kumar N, Bhatia RK. Stepwise bioprocess for exopolysaccharide production using potato starch as carbon source. 3 Biotech. 2015;5:735–739. doi: 10.1007/s13205-014-0273-2. - DOI - PMC - PubMed
1. Bhatia SK, et al. Development of semi-synthetic microbial consortia of Streptomyces coelicolor for increased production of biodiesel (fatty acid methyl esters) Fuel. 2015;159:189–196. doi: 10.1016/j.fuel.2015.06.084. - DOI
1. Bhatia SK, et al. Medium engineering for enhanced production of undecylprodigiosin antibiotic in Streptomyces coelicolor using oil palm biomass hydrolysate as a carbon source. Bioresour Technol. 2016;27:30182–30188. - PubMed

LinkOut - more resources

Full Text Sources
Other Literature Sources
- scite Smart Citations

Save citation to file

Email citation

Add to Collections

Add to My Bibliography

Your saved search

Create a file for external citation management software

Your RSS Feed

Biomass-derived molecules modulate the behavior of Streptomyces coelicolor for antibiotic production

Affiliations

Biomass-derived molecules modulate the behavior of Streptomyces coelicolor for antibiotic production

Authors

Affiliations

Abstract

Conflict of interest statement

Figures

Similar articles

Cited by

References

LinkOut - more resources

Full Text Sources

Other Literature Sources