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. 2016 Dec;6(2):223.
doi: 10.1007/s13205-016-0539-y. Epub 2016 Oct 15.

Biomass-derived molecules modulate the behavior of Streptomyces coelicolor for antibiotic production

Shashi Kant Bhatia  1   2 , Bo-Rahm Lee  1 , Ganesan Sathiyanarayanan  1 , Hun Seok Song  1 , Junyoung Kim  1 , Jong-Min Jeon  1 , Jeong-Jun Yoon  3 , Jungoh Ahn  4 , Kyungmoon Park  5 , Yung-Hun Yang  6   7
Affiliations

Biomass-derived molecules modulate the behavior of Streptomyces coelicolor for antibiotic production

Shashi Kant Bhatia et al. 3 Biotech. 2016 Dec.

Abstract

Various chemicals, i.e., furfural, vanillin, 4-hydroxybenzaldehyde and acetate produced during the pretreatment of biomass affect microbial fermentation. In this study, effect of vanillin, 4-hydroxybenzaldehyde and acetate on antibiotic production in Streptomyces coelicolor is investigated. IC 50 value of vanillin, 4-hydroxybenzaldehyde and acetate was recorded as 5, 11.3 and 115 mM, respectively. Vanillin was found as a very effective molecule, and it completely abolished antibiotic (undecylprodigiosin and actinorhodin) production at 1 mM concentration, while 4-hydroxybenzaldehyde and acetate have little effect. Microscopic analysis with field emission scanning electron microscopy (FESEM) showed that addition of vanillin inhibits mycelia formation and increases differentiation of S. coelicolor cells. Vanillin increases expression of genes responsible for sporulation (ssgA) and decreases expression of antibiotic transcriptional regulator (redD and actII-orf4), while it has no effect on genes related to the mycelia formation (bldA and bldN) and quorum sensing (scbA and scbR). Vanillin does not affect the glycolysis process, but may affect acetate and pyruvate accumulation which leads to increase in fatty acid accumulation. The production of antibiotics using biomass hydrolysates can be quite complex due to the presence of exogenous chemicals such as furfural and vanillin, and needs further detailed study.

Keywords: Antibiotic; Biomass; Furfural; Streptomyces coelicolor; Vanillin.

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Conflict of interest statement

The authors declare that they have no conflict of interest.

Figures

Fig. 1
Fig. 1
Effect of biomass-derived chemicals a vanillin, b 4-hydroxybenzaldehyde (4-HB) and c acetate on S. coelicolor growth and antibiotic production. S. coelicolor was cultured in M9 media with 1 % glucose and various concentrations of inhibitors
Fig. 2
Fig. 2
Field emission scanning electron microscopy (FESEM) of S. coelicolor. a Control: normal cell morphology with mycelia; b with vanillin: cells are round shaped and mycelia formation is inhibited
See this image and copyright information in PMC

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